Anticancer Activity of Modified Tongyou Decoction on Eca109 Esophageal Cancer Cell Invasion and Metastasis through Regulation of the Epithelial-Mesenchymal Transition Mediated by the HIF-1α-Snail Axis

Table 1

RT system.

Reagent	Amount
5 × Hifair®II buffer	4 μL
Hifair®II enzyme mix	2 μL
Oligo (dT)18 (50 μM)	1 μL
Random primer N6 (50 μM)	1 μL
Total RNA	2 μg
RNase-free ddH2O	20 μL (final volume)