Research Article

Effect of Hataedock Treatment on Epidermal Structure Maintenance through Intervention in the Endocannabinoid System

Figure 5

The epidermal structure regulatory effects of HTD. HTD improved the epidermal structures in the FGT group. In toluidine blue staining result, epidermal thickness (arrow (↕) indicates), infiltration lymphocytes (arrow indicates dark navy), and intercellular spaces (arrow indicates white) were increased in the AE group but decreased in the FGT group () (toluidine blue staining; bar size, 50 μm). The E-cadherin expression (arrow indicates dark brown) was decreased in the AE group, but significantly increased in the FGT group () (bar size, 50 μm). The GPx4 expression (arrow indicates dark brown) in the FGT group was remarkably increased compared with the AE group () (bar size, 50 μm). The CD1A expression (arrow indicates dark brown) was significantly increased but decreased in the FGT group (). In TUNEL assay, the apoptotic body (arrow indicates white-blue fluorescence) in the FGT group was remarkably increased compared to the AE group (TUNEL assay; bar size, 50 μm). , compared with the AE group. Ctrl: normal, AE: AD-induced with no treatment, FGT: AD-induced with HTD treatment, EPI: epidermis, DER: dermis, and E-Cad: E-cadherin.