Effect of Hataedock Treatment on Epidermal Structure Maintenance through Intervention in the Endocannabinoid System

Figure 7

The effect of genistein as ECS modulator. (a) Structure of genistein. (b) Expression of involucrin was detected by western blotting using genistein. β-Actin protein was used as an internal control. (c) Effect of genistein on TEWL. Genistein significantly moisturized the skin compared to the DE group (). (d) In western blotting result, the involucrin expression was markedly increased in the GT group (). (e) The expression of involucrin (arrow indicates light brown) was significantly increased in the GT group as compared with the DE group (). The expression of involucrin in the PT group was lower than that in the GT group (bar size, 50 μm). compared with the DE group. Ctrl: normal, DE: dermatitis-induced with no treatment, GT: dermatitis-induced with genistein treatment, PT: dermatitis-induced with PEA treatment, EPI: epidermis, Invol: involucrin, and TEWL: transepidermal water loss.