Shenzhiling Oral Liquid Protects STZ-Injured Oligodendrocyte through PI3K/Akt-mTOR Pathway
An in vitro mode of STZ-induced OLN-93 cell injury was established. (a) The cell viability of OLN-93 cells treated with STZ (0.001 mM–10 mM) for 1–24 h was detected by CCK-8. Each point represents the mean ± SD of n = 6 experiments. (b) Morphological changes of OLN-93 cells under microscope (scale = 100 μm) after treatment with STZ at different concentrations for 3 hours was observed. (c) The relative LDH leakage of OLN-93 cells was determined after treatment with STZ at different concentrations for 3 h. Each point represents the mean ± SD of n = 6 experiments. Changes in IR (d) and IRS1 (e) mRNA transcription levels in OLN-93 cells after STZ treatment for 3 h were observed by qPCR. Mann–Whitney test was used to compare the difference of IR and IRS1 mRNA relative transcript level between control and STZ group. Each point represents the median (IQR) of n = 4 experiments. and , significantly different from control group. IQR means interquartile range.
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