Evidence-Based Complementary and Alternative Medicine

Evidence-Based Complementary and Alternative Medicine / 2020 / Article

Research Article | Open Access

Volume 2020 |Article ID 5172765 | https://doi.org/10.1155/2020/5172765

Ting-Ting Tian, Qi-Rui Li, Shi-Quan Gan, Chu-Rui Chang, Xiang-Chun Shen, "Protective Effect of Simplicillium sp. Ethyl Acetate Extract against High Glucose-Induced Oxidative Stress in HUVECs", Evidence-Based Complementary and Alternative Medicine, vol. 2020, Article ID 5172765, 10 pages, 2020. https://doi.org/10.1155/2020/5172765

Protective Effect of Simplicillium sp. Ethyl Acetate Extract against High Glucose-Induced Oxidative Stress in HUVECs

Academic Editor: Shao-Hsuan Kao
Received26 Mar 2020
Revised15 Jun 2020
Accepted11 Jul 2020
Published17 Aug 2020

Abstract

This study aimed at investigating the cytoprotective effect of an ethyl acetate extract of insect fungi against high glucose- (HG-) induced oxidative damage in human umbilical vein endothelial cells (HUVECs). An insect fungus strain termed CH180672 (CH) was found for protecting HUVECs from HG-induced damage. In this study, CH was identified as Simplicillium sp. based on a phylogenetic analysis of ITS‐rDNA sequences. Ethyl acetate extract (EtOAc) of this strain (CH) was subjected to the following experiments. Cell viability was examined with the MTT method. To evaluate the protection of CH, intracellular reactive oxygen species (ROS), malondialdehyde (MDA) levels, and the activities of antioxidant enzymes were measured and the expression of oxidation-associated proteins was assessed. In the current study, it has been found that CH can increase the survival rate of HUVECs induced by HG. Additionally, we found that HG-induced nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) signal decreased and increased the intracellular ROS and MDA generation in HUVECs. However, CH treatment strongly promoted the translocation of Nrf2 and its transregulation on HO-1 and ultimately inhibited the high level of ROS and MDA induced by HG. The regulatory ability of CH was similar to Nrf2 agonist bardoxolone, while the effect was abolished by ML385, suggesting that Nrf2 mediated the inhibition of CH on HG-induced oxidative stress in HUVECs. Taken together, CH can improve HG-induced oxidative damage of HUVECs, and its mechanism may be related to the regulation of the Nrf2/HO-1 pathway.

1. Introduction

Diabetes mellitus (DM) is an endocrine and metabolic disorder characterized by impaired insulin secretion and increased blood glucose level [1]. Hyperglycemia causes many serious complications such as retinopathy, neuropathy, and nephropathy in diabetic patients [2]. It is notable that hyperglycemia causes a significant increase in reactive oxygen species (ROS) in endothelial cells, leading to cell injury and subsequent cell death [3]. It has been reported that ROS-mediated vascular stress can maintain glucose normalization in DM animals and in cultured endothelial cells. Long-term maintenance of oxidative stress results in the overactivation of pathways closely associated with DM and diabetic complications [4]. Oxidative stress is the initial link and pathological factor of endothelial damage and microvascular diseases. Reducing excessive ROS is crucial for the prevention and treatment of DM complications, especially in endothelial dysfunction and injury.

Nuclear factor-erythroid 2-related factor 2 (Nrf2) is an important antioxidant-regulated transcription factor. Under normal conditions, Nrf2 is constitutively expressed and remains inactive in the cytosol [5]. In response to oxidative stress, Nrf2 is released from its interacting protein and translocates to the nucleus [6, 7], thereby initiating the transcription of substream antioxidant genes, including superoxide dismutase (SOD), catalase, heme-oxygenase 1 (HO-1), and NAD(P)H: quinone oxidoreductase 1 (NQO1) to inactivate the stressors and restore homeostasis [8, 9]. Therefore, the activation of Nrf2 is a key target for the treatment of diabetic microvascular complications and developing new drugs.

Simplicillium belongs to insect fungi which are ancient resources in Chinese medicine. It has been reported that insect fungi have several beneficial biological activities. It has been used for multiple medicinal purposes such as hypoglycemic, hypolipidemic, anti-inflammatory, antitumor, antimetastatic, and antioxidant effects [1013]. In addition, the mycelium of insect fungi has been reported to show strong effects on specific markers of oxidative stress, and its protective effects on heart and blood vessels have been observed in the I/R mouse model [14]. Simplicillium sp. was obtained from insect fungi in Guizhou. The constituent of Simplicillium sp. has never been tested for pharmacological activities. Therefore, we considered whether the Simplicillium sp. (strain CH180672) can reduce HG-induced HUVEC damage and then explore its mechanism.

2. Materials and Methods

2.1. Materials

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), endothelial cell medium (ECM), fetal bovine serum (FBS), penicillin/streptomycin, endothelial cell growth factor, and trypsin neutralization solution were purchased from ScienCell (San Diego, California, USA). 0.25% trypsin-EDTA was purchased from Gibco (Grand Island, NY, USA). 2′,7′-Dichlorofluorescein diacetate DCF-DA was purchased from Beyotime. Primary antibodies Nrf2, HO-1, NQO1, GAPDH, and Lamin B1 were from Proteintech (Wuhan, China). Secondary antibodies including anti-mouse and anti-rabbit were purchased from Bioworld Technology (Minnesota, USA). Bardoxolone and ML385 were purchased from MedChemExpress (Shanghai, China). The specific Nrf2 small-interfering RNA (siRNA) and negative control of siRNA were purchased from GenePharma (Shanghai, China). Enhanced chemiluminescence (ECL) western blotting detection solution was obtained from Beyotime (Shanghai, China).

2.2. Culture and Extraction of Insect Fungi

Insect fungi samples were collected in Guizhou province. Briefly, the fungi were isolated with the method of single-spore isolation. The strains were seeded on potato dextrose agar (PDA) medium and cultured in an incubator at 28°C for 10 days. Agar plugs were cut into small pieces (about 0.5 × 0.5 × 0.5 cm3) under aseptic conditions, and 5 pieces for each were used to inoculate three Erlenmeyer flasks (250 mL), each containing 120 mL of media (NaNO3 3 g/L, K2HPO4 1 g/L, MgSO4·7H2O 0.5 g/L, KCl 0.5 g/L, FeSO4·7H2O 0.01 g/L, D(+)-sucrose 30 g/L, and agar 15 g/L; the final pH of the media was adjusted to 6.5 and the media were sterilized by autoclaving). Three flasks of the inoculated media were incubated at 28°C on a rotary shaker at 120 rpm for five days and then disposed for 15 days. After incubation, the mycelium and fermentation broth were extracted with ethyl acetate (EtOAc), and the extracts were concentrated using a rotary evaporator in a vacuum at 40°C and then freeze-dried at −70°C. The powdered samples (51.2 mg) were dissolved in dimethyl sulfoxide (DMSO) and stored at −20°C until requirement.

2.3. DNA Extraction and ITS Sequencing of the Simplicillium sp. Isolate

The total DNA of the CH was extracted using a Fungal DNA Kit following the manufacturer’s instructions (Fungal DNA Kit; OMEGA Bio-Tek; USA). ITS‐rDNA gene was amplified with primers ITS1/ITS4 (ITS1: 5′‐TCCGTAGGTGAACCTGCGG‐3′ and ITS4: 5′‐TCCTCCGCTTATTGATATGC‐3′). Each PCR was carried out in 25 μL, containing 12.5 μL 2 × HiFi Taq PCR StarMix buffer (GenStar, China), 1 μL of each primer, 1 μL of total DNA, and 9.5 μL of ddH2O. The reaction was then performed using the following reaction cycles: initial denaturation at 94°C for 3 min, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 45 s, and then a final extension phase at 72°C for 10 min. PCR products were visualized by 1.0% agarose gel electrophoresis, stained with Golden View in 1 × TBE buffer, and photographed under UV light. Amplified PCR products were sent to the Beijing Genomics Institute for implementing one‐way sequencing with PCR primers.

2.4. Cell Culture and Screening of Active Strains

HUVECs were cultured in complete medium (ECM, 5% fetal bovine serum, 1% P/S, 1% ECGS) and were incubated in 5% CO2 at 37°C. Screening of active strains was determined using the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay. 1 × 105 cells were seeded in 96-well plates and cotreated with different strains and HG (35 mM) for 24 h, MTT solution was added to each well, and the samples were incubated for 4 hours. The formazan was dissolved by DMSO, and then the optical density was taken at 490 nm using a microplate reader (BioTek Instruments, Vermont, USA).

2.5. Intracellular ROS Analysis

The ROS was determined by a commercial ROS assay kit, using the fluorescent probe DCFH-DA. HUVECs were incubated in 24-well plates with HG (35 mM) in the presence or absence of CH for 24 h, and then cells were washed twice with serum-free medium and stained with 40 µL diluted DCFH-DA at 37°C for 45 min. Intracellular fluorescence was measured at excitation and emission wavelengths of 488 and 525 nm, respectively, and pictures were taken with a fluorescence microscope.

2.6. Detection of Malondialdehyde

The malondialdehyde content (MDA) was measured by the thiobarbituric acid (TBA) method. HUVECs were exposed to HG with or without CH. After 24 hours of administration, cells were washed twice with cold PBS and lysed in RIPA buffer, and released proteins were collected. The remaining steps are carried out according to the manufacturer’s instructions.

2.7. Measurement of Antioxidant Enzyme Activities

HUVECs were cultured in 25 cm3 cell flasks and treated with CH and HG for 24 h, the supernatant was collected, and cells were washed twice with ice-cold PBS and lysed in RIPA buffer. Protein concentrations were determined using the BCA assay. SOD, GSH, and CAT activities were detected using WST-8, Microenzyme label and Visible light methods, especially, according to the manufacturer’s instructions. Experimental assay kits were purchased from Nanjing Jiancheng Institute of Bioengineering, and the experimental steps were performed according to the manufacturer’s instruction.

2.8. Nuclear and Cytoplasmic Extraction

The cytoplasmic and nuclear fractions were separated with extraction reagents. HUVECs were cultured in 25 cm3 cell flasks, treated with CH and HG for 24 h, and then washed thrice with cold PBS. Nuclear protein was extracted using a nuclear and cytoplasmic extraction kit according to the manufacturer’s instructions. The protein concentration was determined by a BCA method. The protein samples were denatured in a sodium dodecyl sulfate (SDS) sample buffer at 100°C for 5 min and then stored at −80°C until further experimentation.

2.9. siRNA Transfection

The manufacturer's instructions were followed. The special sequences of Nrf2-siRNA were as follows: sense: 5′-CCAGAACACUCAGUGGAAUTT-3′ and antisense: 5′-AUUCCACUGAGUGUUCUGGTT-3’. For each transfection, the medium needs to be changed to 1.5% 1640 1 hour in advance, and 22.5 µl siRNA was added to 1125 µl serum-free medium and 1125 µl serum-free medium containing 22.5 µl Lip2000 transfection reagent is gently mixed. After incubating at 37°C for 5 minutes, the complex was added to the cells in 2 ml of transfection medium, and the mixture was incubated at 37°C for 6 hours. The transfection medium was then replaced with normal medium, and the cells were cultured for 24 hours.

2.10. Western Blot Analysis

HUVECs were cultured in 25 cm3 cell flasks, treated with CH and HG for 24 h, washed twice with ice-cold PBS, and lysed in RIPA buffer. Protein concentrations were determined using the BCA assay. Western blotting was conducted by loading 30 µg/mL of protein into the wells of 10% SDS polyacrylamide gels. After separation, proteins were transferred to polyvinylidene difluoride (PVDF) membranes, which were then blocked for 2 h at room temperature in 5% BSA, washed three times with 1 × TBST, and incubated with primary antibodies, anti-Nrf2 (1 : 1000; Proteintech), anti-HO-1 (1 : 1000; Proteintech), anti-NQO1 (1 : 1000; Proteintech), GAPDH (1 : 5000; Proteintech), and Lamin B1 (1 : 5000; Proteintech) overnight at 4°C. Then membranes were washed three times with 1 × TBST for 10 min and incubated with the corresponding secondary antibodies for 1 h at room temperature and then washed three times with 1 × TBST for 10 min. Finally, protein bands were detected by ECL and quantified densitometrically with ImageJ software (ChemiDoc XRS, American Bio-Rad).

2.11. Statistical Analysis

Data were subjected to analysis of variance (ANOVA) using GraphPad Prism® 6.0 software (La Jolla, CA, USA). Statistical analysis was undertaken using ANOVA and t-test for 2 groups and one-way ANOVA for multiple comparisons. Data are expressed as the mean ± SEM. A value of was considered to be statistically significant. Data from in vitro studies were derived from at least 3 independent experiments.

3. Results and Discussion

3.1. Screening Active Strains from Insect Fungi

It is well known that endothelial dysfunction is a key pathological progress of numerous cardiovascular diseases. Therefore, we used HG to establish a HUVEC injury model to screen active strains. The same concentration (32 μg/L) EtOAc extracts from 5 different strains of insect fungi was supplied into HG-treated HUVECs. Results revealed that cell viability percentage was significantly enhanced when treated with HG (35 mM) as compared to the control group. Cell viability percentage of EtOAc extracts from CH180672 and CRW15021008 was significantly increased as compared to the HG-treated group (Figure 1). It indicated that extracts of CH180672 and CRW15021008 have protective effects on HG-induced HUVEC injury. CH180672 could significantly ameliorate the HUVEC injury which was identified as Simplicillium sp. by blasting their ITS sequences on NCBI. In the following experiments, we will focus on CH180672.

3.2. Sequencing and Phylogenetic Analysis of ITS Genes

Here we identified CH180672 as a species of Simplicillium sp. based on phylogenetic analysis. Figure 2 shows that our strain (CH180672) falls in the genus Simplicillium with high support (91/1). Strain CH180672 showed a close relationship with Simplicillium lamellicola (F.E.V. Sm.) Zare & W. Gams. The Simplicillium sp. belongs to insect fungi and is widely used in traditional Chinese herb from ancient times in the treatment of heart and kidney diseases, hyperlipidemia, and hyperglycemia [15, 16].

3.3. CH Ameliorated HG-Induced HUVEC Injury

Firstly, we examined the effect of CH on cell viability in HUVECs. The results of MTT assay showed that CH concentration of 16 and 32 μg/L did not reduce the viability of HUVECs. It indicated that 16 and 32 μg/L CH were nontoxic to HUVECs (Figure 3(a)). We then investigated the effect of CH on HG-induced HUVECs, at both low and high doses. We initially exposed HUVECs to HG (35 mM), and cell damage percentage caused by 35 mM HG was 25% which is in the range of 20%–30%. It indicated that the cell damage model was established successfully. Cell viability percentage was significantly enhanced when treated with 16 µg/L CH () and 32 µg/L CH (), in which pretreatment with CH obviously increased the cell viability of HUVECs in a dose-dependent manner (Figure 3(b)). Our results indicated that CH can ameliorate HG-induced endothelial damage.

3.4. CH Reduced the Generation of ROS in HUVECs Induced by HG

As oxidative stress is crucial for the HG-induced cell injury, it has been reported that decreasing oxidative stress by lowering ROS production is crucial in the management of diabetes complications [17]. We next detected the ROS level in HUVECs. HG treatment produced tremendous fluorescence in HUVECs, suggesting HG-induced oxidative stress to cause endothelial injury. Cotreatment with HG and CH at low and high doses can inhibit increased ROS level induced by HG (Figures 4(a) and 4(b)). Our results proved that CH reduced intracellular ROS accumulation caused by HG.

3.5. CH Reduced the Malondialdehyde (MDA) in HUVECs Induced by HG

ROS can attack unsaturated fatty acids in biofilms and form lipid peroxides. The metabolite malondialdehyde (MDA) is a harmful peroxide. MDA content can directly reflect the degree of lipid peroxidation in cells and indirectly reflect the degree of cell damage. HG increases nonenzymatic glycosylation and results in ROS overproduction, lipid peroxidation, and lipid metabolic disorder. We measured relative rates of lipid peroxidation by measuring levels of MDA, the product of polyunsaturated fatty acid, and related ester breakdown [18]. Figure 5 shows that the level of MDA in the HG group increased significantly compared with the normal group. When pretreated with CH, the level of intracellular MDA was significantly reduced. These data supported that CH may reduce cell damage exposed to HG.

3.6. CH Restored the SOD, CAT, and GSH Levels in HG-Induced HUVECs

After HUVECs were exposed to HG, the activities of SOD and CAT were significantly decreased as well as GSH level. CH could inhibit the decrease of SOD, CAT, and GSH levels (Figures 6(a)6(c)). These results indicated that CH protected oxidative stress damage of HUVECs induced by HG through cellular antioxidation system to control redox balance. Antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) and meanwhile reduced glutathione (GSH) act as defense systems.

However, both oxidant and antioxidant balance are disrupted and cells are damaged by oxidative stress [19]. Our experimental results indicate that HG leads to reduced activity of the endogenous antioxidant enzyme SOD, depleted GSH levels, and decreased cellular CAT while the presence of CH significantly improved their production.

3.7. CH Modulated Nrf2 Translocation and Expression in HG-Induced HUVECs

In the previous study, it was found that cotreatment of HG and CH could quench the overproduction of ROS and MDA induced by HG. Next, the effect of CH on HG-induced HUVECs was investigated at the protein level. Nrf2 is a basic leucine zipper protein that under normal conditions is retained in the cytoplasm [20]. Oxidative stress promotes Nrf2 to translocate into the nucleus where it binds to the AREs in the promoter region of many antioxidant genes such as HO-1 and NAD(P)H dehydrogenase, quinone 1 (NQO1) [21, 22]. Growing evidence indicates that an acute increase in glucose is accompanied by a reduction in endogenous antioxidant genes that may result in endothelial cell dysfunction [23, 24]. In order to explore whether CH plays a protective role through the Nrf2 signaling pathway, we determined whether the increased ROS level may be related to the activation of the antioxidant Nrf2 signal. Western blot analysis found that the protein expression of Nrf2 in the HG model was reduced but increased in HUVECs treated with CH. Similar results were obtained in HO-1 and NQO1 expression by western blot (Figure 7(a)). To further ascertain whether CH treatment altered the Nrf2 nucleus translocation, we isolated nuclear and cytoplasmic proteins of HUVECs in different groups and found that 24 h treatment with CH upregulated the protein abundance of nuclear Nrf2, indicating that Nrf2 was translocated to the nucleus (Figure 7(b)). Our results indicated that CH attenuated oxidative stress and enhanced the nuclear translocation of Nrf2 to stimulate the Nrf2 pathway.

3.8. CH Ameliorated High Glucose-Induced Endothelial Damage via Nrf2 Signal

We hypothesized that Nrf2-mediated CH’s antioxidation function against HG-induced HUVEC injury. To verify this hypothesis, Nrf2 activator bardoxolone and Nrf2 inhibitor ML385 were used, respectively, to regulate Nrf2 signaling activation.

Western blot analysis revealed that Nrf2 and HO-1 protein were significantly downregulated when treated with HG (35 mM) as compared to the control group (). When HUVECs were pretreated with CH (32 μg/L) + bardoxolone (80 nM), we found that Nrf2 and HO-1 protein were upregulated compared with the HG group (Figure 8(a)). However, ML385 abrogated the improvement of CH on Nrf2 and HO-1 protein expression (Figure 8(b)). These results suggested that the protection effect of CH on endothelial cells was depended on the Nrf2/HO-1 signaling activation.

We demonstrated that CH can significantly upregulate Nrf2 expression to attenuate endothelial dysfunction. Moreover, we found that the activator of Nrf2 enhanced the protective function of CH. Meanwhile, we also found that the inhibitor of Nrf2 abolished the protective function of CH. Further evidence from either pharmacological agonist or inhibition revealed that the protective effect of CH on endothelial injury induced by HG occurred through the Nrf2/HO-1 signal pathway.

As shown in Figure 8(a), the experiment used siRNA technology to transfect HUVECs to achieve low expression of Nrf2, and the Nrf2 knockdown efficiency was about 65%. Compared with the HG group, the expression of Nrf2 protein in HUVECs pretreated with CH (32 μg/L) was significantly upregulated. When the expression of Nrf2 was downregulated by siRNA, the role of CH in regulating Nrf2 was weakened. It can be seen from the experimental results that CH improves HG-induced HUVECs damage based on the Nrf2 signaling pathway.

3.9. Identification of the UHPLC-MS Structure of CH180672 Extract

First, it can be judged from the negative ion peak diagram that the CH180672 extract contains compounds (Figure 9), and then the raw data of UPLHC-MS are imported into MS-DIAL 3.82 (MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis. 523–526, 2015). The software performs preprocessing, including peak extraction, denoising, deconvolution, peak alignment, and export of a 3D data matrix (raw data matrix) in CSV format. The extracted peak information is compared with the database MassBank, Respect, GNPS (14951 records in total) three libraries for full library search. This three-dimensional matrix includes information including sample information, retention time, mass-to-nuclear ratio, and mass spectrum response intensity (peak area). From the results, it was preliminarily judged that CH180672 ethyl acetate extract contained chemical components such as adenine, 2′-deoxyadenosine, adenosine, uracil, paeonol, and pilocarpine (Table 1). Studies have shown that adenosine is an endogenous nucleoside with a short half-life, which can regulate the physiological function of the heart and cardiovascular system [25]. In addition, paeonol can prevent tunicamycin-induced vascular endothelial dysfunction by inhibiting endoplasmic reticulum stress and oxidative stress [26]. Chen et al. pointed out that ginsenoside-Rg1 reduces D-galactose-induced oxidative stress in mice and increases the activity of superoxide dismutase and glutathione peroxidase in vivo [27]. Another study showed that magnolol reduced TNF-α-induced vascular endothelial cell damage [28]. Therefore, the components identified in the CH extract have a certain protective effect on vascular endothelial cell damage, which lays a material foundation for its pharmacological activity.


CompoundsAream/z valueAdductionMS1 isotopes

Adenine6719554136.0636[M + H]+117.6775
2′-Deoxyadenosine1039730252.1101[M + H]+251.62872
Adenosine1183380268.106[M + H]+269.0701
Nicotinamide768809123.0558[M + H]+124.08461
Uracil298905.1113.035[M + H]+114.0512
Thymine139144127.0407[M + H]+128.09082
Sinomenine120805.6330.1759[M + H]+332.19388
Paeonol996976.2167.0729[M + H]+168.78964
Pilocarpine237427.4209.1547[M + H]+208.88545
Ginsenoside Rg114236.68845.5034[M + FA − H]845.69629
Magnolol78533.37265.1191[M − H]264.99506
Ginsenoside Rh2 (S-FORM)47211.7667.426[M + FA− H]68.83405

4. Conclusion

In summary, it is the first time to confirm that CH can inhibit HG-induced HUVEC oxidative damage and reveals the potential mechanism involved in the Nrf2/HO-1 signaling pathway and the protective effect of insect fungal extracts of Simplicillium sp. CH inhibits the oxidative damage of HUVECs induced by HG and provides a pharmacological basis for the prevention and treatment of diabetic complications. In addition, it was preliminarily determined that CH180672 ethyl acetate extract contained chemical components, such as adenine, 2′-deoxyadenosine, adenosine, uracil, paeonol, and pilocarpine based on UPLHC-MS results. This provides a certain material basis for the pharmacological effect of CH.

Data Availability

All data used to support the findings of this study are included within the article.

Disclosure

Ting-Ting Tian and Qi-Rui Li are co-first authors.

Conflicts of Interest

The authors confirm that there are no conflicts of interest associated with the publication of this study.

Authors’ Contributions

Chang Chu-Rui and Shen Xiang-Chun designed the study. Tian Ting-Ting performed the experiments. Tian Ting-Ting, Gan Shi-Quan, and Li Qi-Rui analyzed the data and prepared the manuscript. All authors read and approved the final manuscript.

Acknowledgments

This study was supported by the National Natural Science Foundation of China (U1812403-4-4), the Key Project of Guizhou Provincial Natural Science Foundation (no. [2020]1Z069), the Fund of High Level Innovation Talents (grant number 2015-4029), the International Science & Technology Cooperation Base (grant number 2017-5802), the Guiyang Science and Technology Planning Project no. (2017) 30–19, and a grant from Health Commission of Guizhou Province (no. Gzwjkj2019-1-185).

References

  1. M. Napierala, J. Olszewski, I. Miechowicz et al., “The influence of tobacco smoke exposure on selected markers of oxidative stress, kidneys and liver function in the serum of rats with streptozotocin-induced diabetes,” Pharmacological Reports, vol. 71, no. 6, pp. 1293–1298, 2019. View at: Publisher Site | Google Scholar
  2. V. R. Vasanth, S. H. Tan, M. Candasamy, and S. K. Bhattamisra, “Diabetic nephropathy, an update on pathogenesis and drug development,” Diabetes Metabolic Syndrome, vol. 13, no. 1, pp. 754–762, 2019. View at: Publisher Site | Google Scholar
  3. E. Nur, D. P. Brandjes, J.-J. B. Schnog et al., “Plasma levels of advanced glycation end products are associated with haemolysis-related organ complications in sickle cell patients,” British Journal of Haematology, vol. 151, no. 1, pp. 62–69, 2010. View at: Publisher Site | Google Scholar
  4. M. A. Ihnat, J. E. Thorpe, and A. Ceriello, “Hypothesis: the ?metabolic memory?, the new challenge of diabetes,” Diabetic Medicine, vol. 24, no. 6, pp. 582–586, 2007. View at: Publisher Site | Google Scholar
  5. M. Kobayashi, L. Li, N. Iwamoto et al., “The antioxidant defense system Keap1-Nrf2 comprises a multiple sensing mechanism for responding to a wide range of chemical compounds,” Molecular and Cellular Biology, vol. 29, no. 2, pp. 493–502, 2009. View at: Publisher Site | Google Scholar
  6. W. W. Wasserman and W. E. Fahl, “Functional antioxidant responsive elements,” Proceedings of the National Academy of Sciences, vol. 94, no. 10, pp. 5361–5366, 1997. View at: Publisher Site | Google Scholar
  7. A. Raghunath, K. Sundarraj, R. Nagarajan et al., “Antioxidant response elements: discovery, classes, regulation and potential applications,” Redox Biology, vol. 17, pp. 297–314, 2018. View at: Publisher Site | Google Scholar
  8. S. M. Tan and J. B. De Haan, “Combating oxidative stress in diabetic complications with nrf2 activators: how much is too much?” Redox Report, vol. 19, no. 3, pp. 107–117, 2014. View at: Publisher Site | Google Scholar
  9. J. Chen, Z. Zhang, and L. Cai, “Diabetic cardiomyopathy and its prevention by Nrf2: current status,” Diabetes & Metabolism Journal, vol. 38, no. 5, pp. 337–345, 2014. View at: Publisher Site | Google Scholar
  10. S.-Y. Won and E.-H. Park, “Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris,” Journal of Ethnopharmacology, vol. 96, no. 3, pp. 555–561, 2005. View at: Publisher Site | Google Scholar
  11. G. Zhang, Y. Huang, Y. Bian, J. H. Wong, T. B. Ng, and H. Wang, “Hypoglycemic activity of the fungi cordyceps militaris, cordyceps sinensis, tricholoma mongolicum, and omphalia lapidescens in streptozotocin-induced diabetic rats,” Applied Microbiology and Biotechnology, vol. 72, no. 6, pp. 1152–1156, 2006. View at: Publisher Site | Google Scholar
  12. X. Chen, G. Wu, and Z. Huang, “Structural analysis and antioxidant activities of polysaccharides from cultured cordyceps militaris,” International Journal of Biological Macromolecules, vol. 58, pp. 18–22, 2013. View at: Publisher Site | Google Scholar
  13. R. Yu, W. Yang, L. Song, C. Yan, Z. Zhang, and Y. Zhao, “Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured cordyceps militaris,” Carbohydrate Polymers, vol. 70, no. 4, pp. 430–436, 2007. View at: Publisher Site | Google Scholar
  14. X.-F. Yan, Z.-M. Zhang, H.-Y. Yao et al., “Cardiovascular protection and antioxidant activity of the extracts from the mycelia of cordyceps sinensis act partially via adenosine receptors,” Phytotherapy Research, vol. 27, no. 11, pp. 1597–1604, 2013. View at: Publisher Site | Google Scholar
  15. X. K. Xing and S. X. Guo, “The structure and histochemistry of sclerotia of ophiocordyceps sinensis,” Mycologia, vol. 100, no. 4, pp. 616–625, 2008. View at: Publisher Site | Google Scholar
  16. H. S. Tuli, A. K. Sharma, S. S. Sandhu, and D. Kashyap, “Cordycepin: a bioactive metabolite with therapeutic potential,” Life Sciences, vol. 93, no. 23, pp. 863–869, 2013. View at: Publisher Site | Google Scholar
  17. F. M. Amin, R. R. Abdelaziz, M. F. Hamed, M. A. Nader, and G. S. G. Shehatou, “Dimethyl fumarate ameliorates diabetes-associated vascular complications through ROS-TXNIP-NLRP3 inflammasome pathway,” Life Sciences, vol. 256, p. 117887, 2020. View at: Publisher Site | Google Scholar
  18. J. W. Russell, D. Golovoy, A. M. Vincent et al., “High glucose‐induced oxidative stress and mitochondrial dysfunction in neurons,” The FASEB Journal, vol. 16, no. 13, pp. 1738–1748, 2002. View at: Publisher Site | Google Scholar
  19. A. Aminzadeh, “Protective effect of tropisetron on high glucose induced apoptosis and oxidative stress in PC12 cells: roles of JNK, P38 MAPKs, and mitochondria pathway,” Metabolic Brain Disease, vol. 32, no. 3, pp. 819–826, 2017. View at: Publisher Site | Google Scholar
  20. K. Itoh, N. Wakabayashi, Y. Katoh et al., “Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain,” Genes & Development, vol. 13, no. 1, pp. 76–86, 1999. View at: Publisher Site | Google Scholar
  21. A. T. Dinkova-Kostova, W. D. Holtzclaw, R. N. Cole et al., “Direct evidence that sulfhydryl groups of keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants,” Proceedings of the National Academy of Sciences, vol. 99, no. 18, pp. 11908–11913, 2002. View at: Publisher Site | Google Scholar
  22. T. Yamamoto, T. Suzuki, A. Kobayashi et al., “Physiological significance of reactive cysteine residues of keap1 in determining Nrf2 activity,” Molecular and Cellular Biology, vol. 28, no. 8, pp. 2758–2770, 2008. View at: Publisher Site | Google Scholar
  23. K. K. J. Senthil, V. M. Gokila, and S.-Y. Wang, “Activation of Nrf2-mediated anti-oxidant genes by antrodin C prevents hyperglycemia-induced senescence and apoptosis in human endothelial cells,” Oncotarget, vol. 8, no. 57, pp. 96568–96587, 2017. View at: Publisher Site | Google Scholar
  24. S. Wentao, J. Yu, and Q. Kang, “Upregulation of heme oxygenase-1 by brahma-related gene 1 through Nrf2 signaling confers protective effect against high glucose-induced oxidative damage of retinal ganglion cells,” European Journal of Pharmacology, vol. 875, 2020. View at: Publisher Site | Google Scholar
  25. R. Andrejew, T. Glaser, Á. Oliveira-Giacomelli et al., “Targeting purinergic signaling and cell therapy in cardiovascular and neurodegenerative diseases,” Advances in Experimental Medicine and Biology, vol. 1201, pp. 275–353, 2019. View at: Publisher Site | Google Scholar
  26. K.-W. Choy, M. R. Mustafa, Y. S. Lau et al., “Paeonol protects against endoplasmic reticulum stress-induced endothelial dysfunction via AMPK/PPARδ signaling pathway,” Biochemical Pharmacology, vol. 116, pp. 51–62, 2016. View at: Publisher Site | Google Scholar
  27. L. Chen, H. Yao, X. Chen et al., “Ginsenoside Rg1 decreases oxidative stress and down-regulates akt/mTOR signalling to attenuate cognitive impairment in mice and senescence of neural stem cells induced by D-galactose,” Neurochemical Research, vol. 43, no. 2, pp. 430–440, 2018. View at: Publisher Site | Google Scholar
  28. C.-J. Liang, C.-W. Lee, H.-C. Sung et al., “Magnolol reduced TNF-α-induced vascular cell adhesion molecule-1 expression in endothelial cells via JNK/p38 and NF-κB signaling pathways,” The American Journal of Chinese Medicine, vol. 42, no. 03, pp. 619–637, 2014. View at: Publisher Site | Google Scholar

Copyright © 2020 Ting-Ting Tian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder
Views184
Downloads208
Citations

Related articles

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.