Protective Effect of Simplicillium sp. Ethyl Acetate Extract against High Glucose-Induced Oxidative Stress in HUVECs

Figure 4

Effect of CH on intracellular reactive oxygen species (ROS) production in HG-induced HUVECs. (a) Intracellular ROS levels in HUVECs were assessed DCFH-DA probe after treated with high glucose (HG, 35 mM) with or without CH for 24 h. HUVECs were loaded with DCFH-DA in the dark at 37°C for 40 min and then pictures were collected with a fluorescence microscope (magnification 200×). (b) Intracellular fluorescence was measured using a fluorescence microplate at excitation and emission wavelengths of 488 and 525 nm. Results were represented as the mean ± SEM (n = 3). vs. control group; vs. control group; vs. HG group; vs. HG group.

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