Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia by Inhibiting the Nox4/ROS Pathway

Figure 7

Effects of BYHWD on H₂O₂-induced vascular tube formation in HUVECs. (a) Vehicle medium (control) for 10 h. (b) Preincubation with BYHWD (30 mg/ml) for 6 h. (c) H₂O₂ (400 μM) for 10 h. (d) Preincubation with BYHWD (10 mg/ml) for 6 h followed by H₂O₂ for 10 h. (e) Preincubation with BYHWD (20 mg/ml) for 6 h followed by H₂O₂ for 10 h. (f) Preincubation with BYHWD (30 mg/ml) for 6 h followed by H₂O₂ for 10 h. (g) Numbers of vascular tubes in HUVECs cultures for each group and time point. versus the H₂O₂ group at the same time. Scale bars, 100 μm.

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