Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia by Inhibiting the Nox4/ROS Pathway

Figure 8

Effects of BYHWD on H₂O₂-induced intracellular ROS in HUVECs. Cells were stained with DCFH-DA. (a) Vehicle medium (control) for 6 h. (b) Preincubation with BYHWD (30 mg/ml) for 6 h. (c) H₂O₂ (400 μM) for 6 h. (d) Preincubation with BYHWD (10 mg/ml) for 6 h followed by H₂O₂ for 6 h. (e) Preincubation with BYHWD (20 mg/ml) for 6 h followed by H₂O₂ for 6 h. (f) Preincubation with BYHWD (30 mg/ml) for 6 h followed by H₂O₂ for 6 h. (g) Preincubation with apocynin (200 μM) for 1 h followed by H₂O₂ for 6 h. (h) Intracellular ROS level in HUVECs for each group. versus the control group. versus the H₂O₂ group. Scale bars, 50 μm.

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