Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro
Effects of RAD containing serum on caspase-11, tumor necrosis factor-α (TNF-α), and interleukin-3 (IL-3) in Caco-2 cells induced by endotoxin (ET) by ELISA and Western blot. BS: blank serum. RAD-DS: RAD drug-containing serum. ET group: Caco-2 cells were stimulated with 10 EIU/ml of ET, ET + BS group: cells were stimulated with 10 EIU/ml of ET and then cocultured with BS, ET + RAD-CS group: cells were stimulated with 10 EIU/ml of ET and then cocultured with RAD-CS, CK group: control group. (a, c, d) based on ELISA analysis, (b) based on Western blot analysis. Graphs show mean ± SD. Based on one-way ANOVA statistical analysis. ,, and .
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