A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats

Figure 3

ABIN1 is colocated with A20 and NeuN and Iba-1, respectively, in the peri-infarct cortex. (a) Double immunofluorescence staining for ABIN1 and A20 in the peri-infarct cortex at 24 h after reperfusion (n = 3 rats per group). Scale bar = 50 μm. (b) Coimmunoprecipitation of ABIN1 and A20 in the peri-infarct cortex at 24 h after reperfusion (n = 3 rats per group). (c) Double immunofluorescence labeling of ABIN1 (red) and NeuN (green, neurons), Iba-1 (green, microglia), and GFAP (green, astrocytes), respectively (n = 3 rats per group). White arrows show that ABIN1 is colocalized with NeuN and Iba-1, respectively. Scale bar = 50 μm. (d) Comparisons of the percentage of ABIN1⁺ NeuN⁺ cells among ABIN1⁺ cells and ABIN1⁺ Iba-1⁺ cells among ABIN1⁺ cells in the peri-infarct cortex. compared to ABIN1⁺ NeuN⁺/ABIN1⁺.

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