Antipruritic Effect of Ethyl Acetate Extract from Fructus cnidii in Mice with 2,4-Dinitrofluorobenzene-Induced Atopic Dermatitis

Figure 2

Experimental scheme and chronic itch model of AD. (a) Experimental scheme: dorsal neck hair removal on day -5. The 0.5% DNFB was applied to the abdomen (day -3) and the 0.2% DNFB was applied to the dorsal neck (day 0). The animals were sensitized with 0.2% DNFB thrice a week for three weeks (days 0 to 21). EAEFC or tacrolimus was topically applied twice a day for three weeks (days 0 to 21). Chronic scratching behavior was recorded five times on days −2, 1, 8, 15, and 22. (b) In the chronic itch model of AD, the scratching bouts increased after the first week, which can also be seen in Figure 8.

(a)

(b)