Evidence-Based Complementary and Alternative Medicine

Evidence-Based Complementary and Alternative Medicine / 2020 / Article

Research Article | Open Access

Volume 2020 |Article ID 7375862 | https://doi.org/10.1155/2020/7375862

Gui-Nan Shen, Cheng Wang, Ying-Hua Luo, Jia-Ru Wang, Rui Wang, Wan-Ting Xu, Yi Zhang, Yu Zhang, Dong-Jie Zhang, Cheng-Hao Jin, "2-(6-Hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone Induces Apoptosis through ROS-Mediated MAPK, STAT3, and NF-κB Signalling Pathways in Lung Cancer A549 Cells", Evidence-Based Complementary and Alternative Medicine, vol. 2020, Article ID 7375862, 13 pages, 2020. https://doi.org/10.1155/2020/7375862

2-(6-Hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone Induces Apoptosis through ROS-Mediated MAPK, STAT3, and NF-κB Signalling Pathways in Lung Cancer A549 Cells

Academic Editor: Nativ Dudai
Received03 Nov 2019
Revised08 Jun 2020
Accepted08 Jul 2020
Published12 Aug 2020

Abstract

Two novel compounds, 2-(2-hydroxyethylthio)-5,8-dimethoxy-1,4-naphthoquinone (HEDMNQ) and 2-(6-hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone (HHDMNQ), were synthesized to investigate the kill effects and mechanism of 1,4-naphthoquinone derivatives in lung cancer cells. The results of the CCK-8 assay showed that HEDMNQ and HHDMNQ had significant cytotoxic effects on A549, NCI-H23, and NCI-H460 NSCLC cells. Flow cytometry and western blot results indicated that HHDMNQ induced A549 cell cycle arrest at the G2/M phase by decreasing the expression levels of cyclin-dependent kinase 1/2 and cyclin B1. Fluorescence microscopy and flow cytometry results indicated that HHDMNQ could induce A549 cell apoptosis, and western blot analysis showed that HHDMNQ induced apoptosis through regulating the mitochondria pathway, as well as the MAPK, STAT3, and NF-κB signalling pathways. Flow cytometry results showed that intracellular reactive oxygen species (ROS) levels were increased after HHDMNQ treatment, and western blot showed that ROS could modulate the intrinsic pathway and MAPK, STAT3, and NF-κB signalling pathways. These effects were blocked by the ROS inhibitor N-acetyl-L-cysteine in A549 cells. Our findings suggest that compared with HEDMNQ, HHDMNQ had the stronger ability to inhibit the cell viability of lung cancer cells and induce apoptosis by regulating the ROS-mediated intrinsic pathway and MAPK/STAT3/NF-κB signalling pathways. Thus, HHDMNQ might be a potential antitumour compound for treating lung cancer.

1. Introduction

Lung cancer mainly occurs in the bronchial epithelium, and it causes the most cancer-related deaths worldwide [1]. Non-small-cell lung cancer (NSCLC) is a highly metastatic and aggressive lung cancer subtype, accounting for about 80% of lung cancer cases [2]. Standard therapies include surgery, chemotherapy, and molecular-targeted therapy, but the mortality rate for lung cancer patients is still high [35]. Therefore, it is highly desirable to find more effective novel therapeutic targets and develop drugs with high efficacy and low toxicity for NSCLC patients.

Mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, are involved in cancer cell proliferation, migration, and apoptosis [68]. The nuclear factor-κB (NF-κB) signalling pathway includes NF-κB and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α), and has a crucial role in the regulation of cell survival and apoptosis [9]. Signal transducer and activator of transcription 3 (STAT3) is involved in the activation of immunity and apoptosis, and its activation depends on cytokines and growth factors [10]. In addition, some studies have demonstrated that the phosphorylation of MAPKs is associated with activation of the NF-κB signalling pathway [11]. Furthermore, NF-κB and STAT3 are constitutively activated in lung cancer and are promising targets for the development of novel cancer drugs [1214]. Thus, the MAPK, STAT3, and NF-κB signalling pathways may be key targets for cancer therapy.

Reactive oxygen species (ROS) are produced by mitochondrial oxidative phosphorylation and play crucial roles as intracellular messengers in cell proliferation, differentiation, and survival [15]. Oxidative stress can induce excessive production of ROS, leading to cellular damage and apoptosis [16]. Furthermore, ROS play essential roles in maintaining various biological functions by modulating many signal transduction pathways [17]. Therefore, controlling the levels of ROS is important for the treatment of cancer.

The 1,4-naphthoquinone derivatives have been exploited in drug development because of their high anticancer activity and low cytotoxicity [18]. Thus, the anticancer effects of the 1,4-naphthoquinone compound have been the focus of recent studies [1820]. The pharmacophore of 1,4-naphthoquinone imparts anticancer activity in a number of drugs, such as menadione, shikonin, and doxorubicin. The 1,4-naphthoquinone derivatives have been synthesized from natural terpenoids and p-benzoquinones, followed by side chain transformation. Several studies have shown that 1,4-naphthoquinone derivatives with substituent groups at the C-2 site can promote antiproliferative activity [2123].

In this study, we synthesized two novel naphthoquinone derivatives, 2-(2-hydroxyethylthio)-5,8-dimethoxy-1,4-naphthoquinone (HEDMNQ) and 2-(6-hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone (HHDMNQ). Then, we investigated the effects of HEDMNQ and HHDMNQ on cell viability, cell cycle, cell apoptosis, apoptosis-related signalling pathways, and intracellular ROS levels in A549 lung cancer cells.

2. Materials and Methods

2.1. Synthesis of HEDMNQ and HHDMNQ

Aluminum chloride (1.06 M) and sodium chloride (0.484 M) were heated to 150°C, followed by the addition of 1,4-dimethoxybenzene (0.12 M) and maleic anhydride (0.24 M). The mixture was incubated at 170–180°C for 5–10 min. After cooling, 1800 mL of double distilled water (DDW) and 120 mL of 98% hydrogen chloride were added to the mixture, with constant stirring for 12–18 h. Then, naphthazarin was obtained by recrystallizing the filter residue. Naphthazarin (100 mM), butyraldehyde (200 mL), sodium hydrosulfite (60 mM), DDW (200 mL), and tetrabutylammonium bromide (2 g) were stirred for 2 h at room temperature, and a mixture of sodium dithionite (10.6 g), 50% sodium hydroxide solution (40 mL), and dimethyl sulfate (25 mL) was added every 3 h. After constant stirring for 12–18 h, 1,4,5,8-tetramethoxynaphthalene was generated and dissolved in acetonitrile (450 mL) and chloroform (150 mL). Ceric ammonium nitrate (54 g) was added to the solution and stirred for 0.5–1 h, and 5,8-dimethoxy-1,4-naphthoquinone was obtained by recrystallizing. Next, 2-hydroxy-1-ethanethiol (1.5 mM) and 6-mercapto-1-hexanol (1.5 mM) were added to a solution of 5,8-dimethoxy-1,4-naphthoquinone (1 mM) in MeOH (30 mL). The mixture was stirred for 4 h at room temperature and stirred for 5 min with sodium dichromate (0.6 mM) and sulfuric acid (0.6 mM). Two novel compounds, HEDMNQ and HHDMNQ, were separated by chromatography. Nuclear magnetic resonance (NMR) spectra were recorded on a JNM-AL 400 (400 MHz) spectrometer. Chemical shifts (d) are given in ppm downfield from tetramethylsilane as the internal standard. Mass spectra were collected with the AB SCIEX API 2000 LC/MS/MS (Applied Biosystems, Inc., Foster City, CA, USA) and LCMS-IT-TOF (Shimadzu Scientific Inc., Beijing, China) systems.

2.2. Cell Culture

Human lung cancer cell lines A549, NCI-H23, and NCI-H460 were obtained from the American Type Culture Collection (Manassas, VA, USA); and normal lung IMR-90, liver L-02, and stomach GES-1 cell lines were purchased from Saiqi Biological Engineering Co., Ltd. (Shanghai, China). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and grown at 37°C in a humidified atmosphere with 5% CO2.

2.3. Cell Counting Kit-8 Assay Determination of Cell Viabilities

Briefly, the human lung cancer A549, NCI-H23, and NCI-H460 cells and normal lung IMR-90, liver L-02, and stomach GES-1 cell lines were counted and seeded into 96-well culture plates followed by adherence for 24 h. Then, the cells were treated with different concentrations of shikonin, fluorouracil (5-FU), HEDMNQ, and HHDMNQ (0, 1, 3, 10, 30, and 100 μM) for 24 h. Followed by starvation for 2 h, 10 μL of CCK-8 solution (Beyotime Biotechnology, Beijing, China) was added to each well. After incubation for 1 h at 37°C, the absorbance of the solution was measured at 450 nm with an automatic microplate reader (BioTek Instruments, Inc., Winooski, VT).

In addition, the A549, NCI-H23, NCI-H460, IMR-90, L-02, and GES-1 cells were seeded in 96-well plates. According to the IC50 value of shikonin, 5-FU, HEDMNQ, and HHDMNQ in human lung cancer and normal cell lines, the cells were treated with HEDMNQ and HHDMNQ for 3, 6, 12, 24, and 36 h. Next, 10 μL of CCK-8 solution was added to the cells and measured by using an automatic microplate reader.

2.4. Flow Cytometry Determination of Cell Cycle

A549 cells were incubated with 6 μM HHDMNQ for the indicated times (3, 6, 12, and 24 h), and the cells were harvested and fixed in 70% ethanol at 4°C for 24 h. Cell suspensions were resuspended in 195 μL binding buffer and incubated with RNase and propidium iodide (PI) (Beyotime Biotechnology) for 30 min in the dark at 37°C. Then, the cells were analyzed by flow cytometry (Beckman Coulter, Brea, CA, USA) with a 488 nm argon laser.

2.5. Fluorescence Microscopy Determination of Apoptosis

Briefly, 4 × 104 cells/well of A549 cells were cultured onto 6-well plates followed by adherence overnight and subsequent treatment with 6 μM HHDMNQ for the indicated times (3, 6, 12, and 24 h). Then, the cells were harvested and suspended in phosphate-buffered saline (PBS), after which the cell suspensions were stained with Hoechst 33342 and PI (Beyotime Biotechnology) for 15 min in the dark and analyzed by fluorescence microscopy (EVOS FL, Life technology, USA).

2.6. Flow Cytometry Determination of Apoptosis

A549 cells were incubated with 6 μM HHDMNQ for the indicated times (3, 6, 12, and 24 h), and the cells were harvested and resuspended in PBS. Then, the cell suspensions were stained with Annexin V-FITC and PI (Beyotime Biotechnology) for 15 min in the dark and analyzed by flow cytometry (Beckman Coulter).

2.7. Flow Cytometry Determination of ROS

Many studies have shown that DCFH-DA staining is one of the most widely used techniques for directly measuring the redox state of cells. A549 cells were incubated with 6 μM HHDMNQ for the indicated times (3, 6, 12, and 24 h), and incubated with 10 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Merck, Shanghai, China) for 30 min at 37°C. Then, the cells were centrifuged at 5,000 × for 5 min, washed, and resuspended in PBS. 2′,7′-Dichlorofluorescein fluorescence was analyzed by flow cytometry.

2.8. Western Blot Analysis of Proteins

A549 cells were harvested and lysed in lysis buffer. The supernatant was collected after centrifugation at 12,000 × for 30 min at 4°C and dissolved at 30 μg proteins per 20 μL with DDW and 5× buffer. The proteins were separated on 10% SDS-PAGE gels and electrotransferred onto nitrocellulose membranes. Then, the membranes were blocked in 5% skim milk and incubated with primary antibodies overnight at 4°C. After incubation with secondary antibodies, the protein bands were detected using enhanced chemiluminescence detection reagents. The intensity of the bands was determined using the ImageJ software.

2.9. Statistical Analysis

The results were analyzed by comparison between different groups. The IC50 was calculated by the GraphPad Prism 5.0 software. The continuous data were analyzed by one-way analysis of variance followed by Tukey’s post-hoc tests using the SPSS 18.0 statistical software. Statistically significant values were defined as . All of the experiments were replicated three times and presented as mean ± standard deviation (SD).

3. Results

3.1. Synthesis of the 1,4-Naphthoquinone Derivatives HEDMNQ and HHDMNQ

To improve anticancer activity and reduce the side effects of 1,4-naphthoquinone, we synthesized HEDMNQ and HHDMNQ (Figure 1). By performing NMR at a wavelength of 400 MHz, we analyzed the H, C and mass spectra in deuterated chloroform solvent and identified the following structures.

HEDMNQ : 1H-NMR (CDCl3, 400 MHz): δ 7.34 (d, J = 9.6 Hz, 1H), 7.28 (d, J = 9.6 Hz, 1H), 6.61 (s, 1H), 3.96 (s, 6H), 3.93 (t, J = 6.4 Hz, 2H), 3.05 (t, J = 6.4 Hz, 2H); 13C NMR (CDCl3, 150 MHz): δ 181.8 (C-1), 181.6 (C-4), 154.3 (C-5), 153.6 (C-8), 153.5 (C-2), 127.8 (C-3), 123.3 (C-7), 121.0 (C-6), 120.7 (C-10), 119.7 (C-9), 56.8 (OCH3), 56.8 (OCH3), 59.7 (C-1′), 31.9 (C-2′); m/z 316.9 (M + Na)+.

HHDMNQ : 1H-NMR (CDCl3, 400 MHz): δ 7.33 (d, J = 9.6 Hz, 1H), 7.27 (d, J = 9.6 Hz, 1H), 6.45 (s, 1H), 3.96 (s, 6H), 3.66 (t, J = 6.4 Hz, 2H), 2.76 (t, J = 7.6 Hz, 2H), 1.78–1.72 (m, 2H), 1.61–1.25 (m, 6H); 13C NMR (CDCl3, 150 MHz): δ 181.76 (C-1), 181.74 (C-4), 154.4 (C-5), 154.3 (C-8), 153.7 (C-2), 127.5 (C-3), 121.0 (C-7), 120.91 (C-6), 120.91 (C-10), 119.6 (C-9), 56.8 (OCH3), 56.5 (OCH3), 56.9 (C-1′), 32.3 (C-2′), 31.9 (C-3′), 31.4 (C-4′), 30.4 (C-5′), 30.2 (C-6′); m/z 372.9 (M + Na)+.

3.2. HEDMNQ and HHDMNQ Inhibit Lung Cancer Cell Proliferation

To determine the cell cytotoxicity after HEDMNQ and HHDMNQ treatment, cell viabilities were measured by using the CCK-8 assay. As shown in Figures 2(a) and 2(c), HEDMNQ and HHDMNQ significantly inhibited the growth of A549, NCI-H23, and NCI-H460 non-small-cell lung cancer cells in a dose- and time-dependent manner, and the IC50 values of Shikonin, 5-FU, HEDMNQ, and HHDMNQ for each cell line are shown in Table 1. As shown in Figures 2(b) and 2(d), HEDMNQ and HHDMNQ had significantly lower cytotoxicity compared with shikonin and 5-FU in normal lung IMR-90, liver L-02, and stomach GES-1 cells. These data indicated that HEDMNQ and HHDMNQ selectively inhibited the viability of lung cancer cells with less cytotoxic effects on nonmalignant cells. In addition, HHDMNQ had the stronger inhibitory effects on cell viability, and compared with other cells, the A549 cells have a lower IC50 value and more sensitive to HHDMNQ, so we chose them for further study.


Cell nameShikonin (μM)5-FU (μM)HEDMNQ (μM)HHDMNQ (μM)

A54952.83 ± 2.0252.83 ± 2.0210.91 ± 1.225.62 ± 2.16
NCI-H2371.55 ± 2.3569.31 ± 3.3914.78 ± 2.316.62 ± 1.65
NCI-H46079.19 ± 2.1274.14 ± 1.8414.22 ± 2.069.07 ± 1.38

3.3. HHDMNQ Induces Cell Cycle Arrest at the G2/M Phase in A549 Cells

To assess the effect of HHDMNQ on cell cycle distribution, A549 cells were treated with HHDMNQ for 3, 6 12, and 24 h. The percentage of cells at the G0/G1 phase was decreased and that at the G2/M phase was increased in a time-dependent manner. Next, we detected the G2/M phase-related proteins to evaluate HHDMNQ-induced cell cycle arrest (Figure 3(a)). The expression levels of p21 and p27 were increased, and those of cyclin B1 and cyclin-dependent kinase 1/2 (CDK1/2) were decreased (Figure 3(b)). These results suggested that HHDMNQ arrested the cell cycle at the G2/M phase concomitantly with the downregulation of cyclin B1 and CDK1/2 in A549 cells.

3.4. HHDMNQ Induces Apoptosis in A549 Cells

To assess whether the suppressed cell viability effects of HHDMNQ were associated with apoptosis, A549 lung cancer cells were stained with Hoechst 33342 and PI. As shown in Figure 4(a), after treatment with HHDMNQ, nuclear chromatin condensation and fragmented punctuate blue and red nuclear fluorescence were observed in A549 cells in a time-dependent manner, similar to morphological changes in the apoptotic cells. To further assess the apoptosis caused by HHDMNQ, the apoptosis rate of A549 cells was examined by flow cytometry. HHDMNQ triggered the apoptosis of A549 cells, starting at 12 h, in a time-dependent manner (Figure 4(b)). These results suggested that HHDMNQ could induce the apoptosis of lung cancer A549 cells. Since HHDMNQ could induce apoptosis of A549 cells, we examined apoptosis-related proteins by western blot. As shown in Figure 4(c), the expression levels of Bcl-2-associated X protein (Bax), Bcl-2-associated death promoter (Bad), cleaved caspase-3, and cleaved PARP were increased after treatment with HHDNMQ, whereas the expression levels of the anti-apoptotic proteins B-cell lymphoma 2 (Bcl-2), B-cell lymphoma-extra-large (Bcl-xL), pro-caspase-3, pro-poly (ADP-ribose) polymerase (PARP), and Bcl-2/Bax ratio were significantly decreased in a time-dependent manner. These results illustrated that HHDMNQ activated the mitochondria-dependent pathway to induce apoptosis in A549 lung cancer cells.

3.5. HHDMNQ Regulates the MAPK, STAT3, and NF-κB Signalling Pathways in A549 Cells

To investigate the underlying mechanisms of HHDMNQ-induced apoptosis in lung cancer cells, the key proteins in the MAPK, STAT3, and NF-κB signalling pathways were examined. As shown in Figures 5(a)5(e), HHDMNQ treatment inhibited the activation of p-ERK, p-STAT3 (Tyr 705) and NF-κB (p65) but promoted the expression of p-p38, p-JNK, and IκB-α in a time-dependent manner. These results indicated that regulating MAPK, STAT3, and NF-κB activity may promote HHDMNQ-mediated induction of cell apoptosis in lung cancer.

3.6. HHDMNQ Induces Oxidative Stress-Mediated Apoptosis in A549 Cells

To determine whether intracellular ROS levels are associated with activation of the programmed cell apoptosis mechanism in lung cancer cells, the levels of ROS and cell apoptosis were determined. As shown in Figure 6(a), the levels of ROS were significantly increased in a time-dependent manner after treating A549 cells with HHDMNQ for 3, 6, 12, and 24 h. As shown in Figure 6(b), intracellular ROS levels were suppressed after pretreatment with the specific ROS inhibitor NAC. To demonstrate whether HHDMNQ-induced increases in ROS were associated with the apoptosis of A549 cells, cell apoptosis-related proteins were determined. As shown in Figure 6(c), NAC significantly reversed the increased expression levels of Bax, Bad, cleaved caspase-3, and cleaved PARP and the decreased the expression levels of Bcl-2, Bcl-xL, pro-caspase-3, and pro-PARP. These findings demonstrated that HHDMNQ increased intracellular ROS levels, resulting in A549 cell apoptosis.

3.7. HHDMNQ Induces Apoptosis through ROS-Mediated MAPK, STAT3, and NF-κB Signalling Pathways in A549 Cells

To demonstrate the underlying molecular mechanism of HHDMNQ-induced ROS-mediated apoptosis of A549 cells, we examined the effects of HHDMNQ on MAPK, STAT3, and NF-κB signalling pathways that play pivotal roles in cancer cells. As shown in Figures 7(a)7(e), the expression levels of p38, JNK, and IκB-α were increased and those of ERK, STAT3, and NF-κB were decreased after HHDMNQ treatment. However, the expression levels of proteins were reversed in the HHDMNQ and NAC treatment groups. These results indicated that HHDMNQ induced A549 cell apoptosis by regulating ROS-mediated MAPK, STAT3, and NF-κB signalling pathways.

4. Discussion

There is an urgent need to develop novel anticancer drugs, and inhibition of proliferation and induction of apoptosis in cancer cells are the potential therapeutic strategies for drug development [24]. The 1,4-naphthoquinone pharmacophore has growth inhibitory activity and can induce apoptosis and anti-inflammation in various cancer cell lines. Furthermore, the side chain can influence the entire activity of 1,4-naphthoquinone derivatives [25]. In this study, we used 1,4-naphthoquinone as a pharmacophore to synthesize the novel naphthoquinone derivatives HEDMNQ and HHDMNQ. The 1,4-naphthoquinone derivatives suppressed the growth of lung cancer cells with the lower IC50 values and lower cytotoxicity in normal cell lines. HHDMNQ had higher efficiency and lower cytotoxicity than HEDMNQ. Furthermore, HHDMNQ induced cell cycle arrest at the G2/M phase by regulating the CDK/cyclin complex in A549 lung cancer cells.

Induction of cell apoptosis is a crucial physiological process to inhibit cancer cell proliferation. In addition, cell apoptosis is related to the promotion of pro-apoptotic proteins and the suppression of anti-apoptotic proteins, and the Bcl-2 family is foremost in regulating the mitochondrial pathway [26]. In this study, fluorescence microscopy showed A549 cell shrinkage, deformation, and shedding; and flow cytometry results showed that early apoptotic and late apoptotic cells were increased after HHDMNQ treatment in a time-dependent manner. Furthermore, HHDMNQ promoted the downregulation of Bcl-2 and Bcl-xL and the upregulation of Bad, Bax, cleaved caspase-3, and cleaved PARP in A549 cells. Thus, it can be considered that HHDMNQ-mediated induction of A549 lung cancer cell apoptosis is associated with regulation of the mitochondria-regulated apoptosis pathway. In addition, some studies suggested that the mutant p53 could promote cancer cell proliferation, invasion, and drug resistance, and the wild-type p53 could mediate various cellular activities, such as promote cell cycle arrest and apoptosis by inhibiting the phosphorylation of STAT3 and regulating the expression of cell cycle target genes [27, 28]. It has been shown that 2-(naphthalene-2-thio)-5,8-dimethoxy-1,4-naphthoquinone could induce G0/G1 phase arrest by regulating the wild-type p53 in cancer cells [29]. The structures of 2-(naphthalene-2-thio)-5,8-dimethoxy-1,4-naphthoquinone are similar to the compound that we synthesized. On the basis of the above, we speculate that HHDMNQ induced cell apoptosis and G2/M cell cycle arrest may be related to p53 expression.

Developing anticancer agents that modulate inflammatory signalling pathways and cell proliferation may be a rational strategy for preventing or treating lung cancers. It has been demonstrated that ERK, JNK, and p38 are the three main components of the MAPK signalling pathway, and they play an important regulatory role in cancer cell apoptosis [30]. ERK is a key protein kinase in the MAPK family, whose abnormal activation will cause the proliferation and invasion of various tumor cells. The results indicated that paxillin promoted Bcl-2 activation by mediating ERK activation, which was associated with tumor formation efficacy in mice [31]. The Janus kinase (JAK)/STAT signalling pathway is one of the important signalling pathways in modulating cell proliferation, differentiation, migration, and apoptosis [32]. STAT3 maintains an abnormal activation state, which can accelerate the cycle process by controlling the protein levels of cyclin D, p21, and p27, promoting the new blood vessels generation and providing supplementary nutrition for the development of cancer cells. Some studies suggested that celecoxib induced apoptosis and cell cycle arrest via inhibition of STAT3 phosphorylation in NPC cells [33, 34]. The NF-κB signalling pathway is associated with the growth and survival of cancer cells [35]. IκB-α is an important factor in the NF-κB family of inhibitors, whose phosphorylation is closely related to NF-κB activation. The phosphorylation of IκB-α will result in the ubiquitin-proteasome system degradation, producing amounts of NF-κB dimers, while inhibition of the phosphorylation leads to the inactivation of NF-κB. And the inhibition effects on NF-κB activation caused the ratio of Bax/Bcl-2 upregulated in the Bcl-2 family and then promoted the activation of caspase-3 [36, 37]. In this study, we examined whether HHDMNQ treatment regulates the MAPK, STAT3, and NF-κB signalling pathways. We found that HHDMNQ efficiently promoted the phosphorylation of p38 and JNK and inhibited the phosphorylation of ERK, STAT3, NF-κB and IκB-α in a time-dependent manner. The MAPK, STAT3 and NF-κB signalling pathways were inhibited by HHDMNQ, and they were closely associated with the suppression of proliferation and induction of A549 lung cancer cell apoptosis.

Oxidative stress regulates cancer cell apoptosis by activating multifarious redox signalling cascade reactions [38]. The mitochondrial pathway is the main component of the intrinsic apoptosis mechanism, and it is the main target of ROS. The Bcl-2 family of proteins and caspases is the characteristic feature of ROS-mediated mitochondrial-induced apoptosis in cancer cells [39]. Numerous small-molecule compounds have been shown to stimulate excessive ROS production, and they are associated with the induction of cell apoptosis. In this study, HHDMNQ promoted the accumulation of ROS in A549 cells in a time-dependent manner, and pretreatment with NAC restored the levels of ROS. HHDMNQ induced cell apoptosis, and the expression levels of apoptosis regulators were completely reversed by NAC pretreatment. We hypothesized that HHDMNQ could modulate MAPK, STAT3, and NF-κB signalling pathway activation through regulating intracellular ROS levels to induce A549 cell apoptosis. We confirmed our hypotheses by using NAC, which reversed the expression levels of MAPK, STAT3, and NF-κB signalling-pathway-related proteins in A549 cells. Pretreatment of A549 cells with NAC not only abolished the effects of HHDMNQ-induced apoptosis but also reversed HHDMNQ-mediated suppressive effects on MAPK, STAT3, and NF-κB signalling pathways in A549 cells.

5. Conclusion

In conclusion, we synthesized the two novel types of 1,4-naphthoquinone derivatives HEDMNQ and HHDMNQ. This study revealed for the first time that HHDMNQ induced mitochondria-dependent apoptosis and G2/M cell cycle arrest promoted the accumulation of ROS and mediated MAPK/STAT3/NF-κB signalling pathways and then regulated the downstream-related apoptosis and cycle proteins in A549 lung cancer cells (Figure 8).

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Authors’ Contributions

Gui-Nan Shen, Cheng Wang, and Ying-Hua Luo contributed equally to this study as first authors.

Acknowledgments

This work was supported by the Postdoctoral Scientific Research Foundation of Heilongjiang Province of China (Grant No. LBH-Q13132), the Heilongjiang Farms and Land Reclamation Administration Support Project for Key Scientific Research (No. HKKYZD190705), the Heilongjiang Bayi Agricultural University Support Program for “San Zong” (No. TDJH201905), the Heilongjiang Touyan Innovation Team Program (No. 2019HTY078), and Research Project of Heilongjiang Bayi Agricultural University (No. XDB202012).

References

  1. A. Mathew, “Global survey of clinical oncology workforce,” Journal of Global Oncology, vol. 4, pp. 4–12, 2018. View at: Google Scholar
  2. S. C. Tripathi, J. F. Fahrmann, M. Celiktas et al., “MCAM mediates chemoresistance in small-cell lung cancer via the PI3K/AKT/SOX2 signaling pathway,” Cancer Research, vol. 77, no. 16, pp. 4414–4425, 2017. View at: Publisher Site | Google Scholar
  3. T. N. Zamay, G. S. Zamay, O. S. Kolovskaya et al., “Current and prospective protein biomarkers of lung cancer,” Cancers (Basel), vol. 9, no. 11, pp. 155–176, 2017. View at: Publisher Site | Google Scholar
  4. O. Valencia, G. Lopes, P. Sánchez, L. Acuña, D. Uribe, and J. González, “Incidence and prevalence of cancer in Colombia: the methodology used matters,” Journal of Global Oncology, vol. 4, no. 4, pp. 1–7, 2018. View at: Publisher Site | Google Scholar
  5. F. Bray, J. Ferlay, I. Soerjomataram, R. L. Siegel, L. A. Torre, and A. Jemal, “Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA: A Cancer Journal for Clinicians, vol. 68, no. 6, pp. 394–424, 2018. View at: Publisher Site | Google Scholar
  6. P. Xie, F. Horio, I. Fujii, J. Zhao, M. Shinohara, and M. Matsukura, “A novel polysaccharide derived from algae extract inhibits cancer progression via JNK, not via the p38 MAPK signaling pathway,” International Journal of Oncology, vol. 52, no. 5, pp. 1380–1390, 2018. View at: Google Scholar
  7. D. Li, Y. Wei, S. Xu et al., “A systematic review and meta-analysis of bidirectional effect of arsenic on ERK signaling pathway,” Molecular Medicine Reports, vol. 17, no. 3, pp. 4422–4432, 2018. View at: Google Scholar
  8. F. Gao and W. J. Liu, “Advance in the study on p38 MAPK mediated drug resistance in leukemia,” European Review for Medical and Pharmacological Sciences, vol. 20, no. 6, pp. 1064–1070, 2016. View at: Google Scholar
  9. L. Yang, Y. Zhou, Y. Li et al., “Mutations of p53 and KRAS activate NF-κB to promote chemoresistance and tumorigenesis via dysregulation of cell cycle and suppression of apoptosis in lung cancer cells,” Cancer Letters, vol. 357, no. 2, pp. 520–526, 2015. View at: Publisher Site | Google Scholar
  10. A. Maryam, T. Mehmood, H. Zhang et al., “Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress,” Science Reports, vol. 7, no. 1, p. 6242, 2017. View at: Publisher Site | Google Scholar
  11. K. Szoltysek, A. Walaszczyk, P. Janus, M. Kimmel, and P. Widlak, “Irradiation with UV-C inhibits TNF-α-dependent activation of the NF-κB pathway in a mechanism potentially mediated by reactive oxygen species,” Genes to Cells, vol. 22, no. 1, pp. 45–58, 2017. View at: Publisher Site | Google Scholar
  12. M.-H. Cheng, Y.-H. Wong, C.-M. Chang et al., “B1, a novel HDAC inhibitor, induces apoptosis through the regulation of STAT3 and NF-κB,” International Journal of Molecular Medicine, vol. 39, no. 5, pp. 1137–1148, 2017. View at: Publisher Site | Google Scholar
  13. D. Li, J. Sun, W. Liu et al., “Rig-G is a growth inhibitory factor of lung cancer cells that suppresses STAT3 and NF-κB,” Oncotarget, vol. 7, no. 40, pp. 66032–66050, 2016. View at: Publisher Site | Google Scholar
  14. T. Mehmood, A. Maryam, X. Tian, M. Khan, and T. Ma, “Santamarine inhibits NF-кB and STAT3 activation and induces apoptosis in HepG2 liver cancer cells via oxidative stress,” Journal of Cancer, vol. 8, no. 18, pp. 3707–3717, 2017. View at: Publisher Site | Google Scholar
  15. X. J. Shi, B. Yu, J. W. Wang et al., “Structurally novel steroidal spirooxindole by241 potently inhibits tumor growth mainly through ROS-mediated mechanisms,” Science Reports, vol. 6, p. 31607, 2016. View at: Publisher Site | Google Scholar
  16. M. H. Raza, S. Siraj, A. Arshad et al., “ROS-modulated therapeutic approaches in cancer treatment,” Journal of Cancer Research and Clinical Oncology, vol. 143, no. 9, pp. 1789–1809, 2017. View at: Publisher Site | Google Scholar
  17. Z. Zou, H. Chang, H. Li, and S. Wang, “Induction of reactive oxygen species: an emerging approach for cancer therapy,” Apoptosis, vol. 22, no. 11, pp. 1321–1335, 2017. View at: Publisher Site | Google Scholar
  18. S.-H. Wang, C.-Y. Lo, Z.-H. Gwo et al., “Synthesis and biological evaluation of lipophilic 1,4-naphthoquinone derivatives against human cancer cell lines,” Molecules, vol. 20, no. 7, pp. 11994–12015, 2015. View at: Publisher Site | Google Scholar
  19. S. K. Ghosh, A. Ganta, and R. A. Spanjaard, “Discovery and cellular stress pathway analysis of 1,4-naphthoquinone derivatives with novel, highly potent broad-spectrum anticancer activity,” J Biomed Sci, vol. 25, no. 1, p. 12, 2018. View at: Publisher Site | Google Scholar
  20. W.-T. Xu, G.-N. Shen, Y.-H. Luo et al., “New naphthalene derivatives induce human lung cancer A549 cell apoptosis via ROS-mediated MAPKs, Akt, and STAT3 signaling pathways,” Chemico-Biological Interactions, vol. 304, pp. 148–157, 2019. View at: Publisher Site | Google Scholar
  21. C. Liu, G.-N. Shen, Y.-H. Luo et al., “Novel 1,4-naphthoquinone derivatives induce apoptosis via ROS-mediated p38/MAPK, Akt and STAT3 signaling in human hepatoma Hep3B cells,” The International Journal of Biochemistry & Cell Biology, vol. 96, pp. 9–19, 2018. View at: Publisher Site | Google Scholar
  22. J.-R. Wang, G.-N. Shen, Y.-H. Luo et al., “The compound 2-(naphthalene-2-thio)-5,8-dimethoxy-1,4-naphthoquinone induces apoptosis via reactive oxygen species-regulated mitogen-activated protein kinase, protein kinase B, and signal transducer and activator of transcription 3 signaling in human gast,” Drug Development Research, vol. 79, no. 6, pp. 295–306, 2018. View at: Publisher Site | Google Scholar
  23. Y. Wang, Y. H. Luo, X. J. Piao et al., “Novel 1,4-naphthoquinone derivatives induce reactive oxygen species-mediated apoptosis in liver cancer cells,” Molecular Medicine Reports, vol. 19, no. 3, pp. 1654–1664, 2019. View at: Google Scholar
  24. R. McCall, M. Miles, P. Lascuna et al., “Dual targeting of the cancer antioxidant network with 1,4-naphthoquinone fused gold(i) N-heterocyclic carbene complexes,” Chemical Science, vol. 8, no. 9, pp. 5918–5929, 2017. View at: Publisher Site | Google Scholar
  25. Y. Zhang, Y.-H. Luo, X.-J. Piao et al., “The design of 1,4-naphthoquinone derivatives and mechanisms underlying apoptosis induction through ROS-dependent MAPK/Akt/STAT3 pathways in human lung cancer cells,” Bioorganic & Medicinal Chemistry, vol. 27, no. 8, pp. 1577–1587, 2019. View at: Publisher Site | Google Scholar
  26. M. H. Hodroj, A. Jardaly, S. Abi Raad, A. Zouein, and S. Rizk, “Andrographolide potentiates the antitumor effect of topotecan in acute myeloid leukemia cells through an intrinsic apoptotic pathway,” Cancer Management and Research, vol. 10, pp. 1079–1088, 2018. View at: Publisher Site | Google Scholar
  27. D. Zhao, W. M. Tahaney, A. Mazumdar, M. I. Savage, and P. H. Brown, “Molecularly targeted therapies for p53-mutant cancers,” Cellular and Molecular Life Sciences, vol. 74, no. 22, pp. 4171–4187, 2017. View at: Publisher Site | Google Scholar
  28. J. Liu and C. Zhang, “Tumor suppressor p53 and its gain-of-function mutants in cancer,” Acta Biochimica et Biophysica Sinica, vol. 46, no. 3, pp. 170–179, 2014. View at: Publisher Site | Google Scholar
  29. Y. Feng, Y. H. Luo, S. M. Li et al., 2-(Naphthalene-2-Thio)-5,8-Dimethoxy-1,4-Naphthoquinone Induces Apoptosis Via Ros-Mediated Mapk, Akt, And Stat3 Signaling Pathways In Hepg2 Human Hepatocellular Carcinoma Cells, Taylor & Francis, Abington, UK, 2019.
  30. S.-G. Park, S.-H. Kim, K.-Y. Kim et al., “Toyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells,” Pharmacological Reports, vol. 69, no. 1, pp. 90–96, 2017. View at: Publisher Site | Google Scholar
  31. C. Zhang, Y. Yu, Q. Huang, and K. Tang, “SIRT6 regulates the proliferation and apoptosis of hepatocellular carcinoma via the ERK1/2 signaling pathway,” Molecular Medicine Reports, vol. 20, no. 2, pp. 1575–1582, 2019. View at: Google Scholar
  32. Y. Li, W. Sun, N. Han et al., “Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway,” BMC Cancer, vol. 18, no. 1, p. 1230, 2018. View at: Publisher Site | Google Scholar
  33. D.-b. Liu, G.-y. Hu, G.-x. Long, H. Qiu, Q. Mei, and G.-q. Hu, “Celecoxib induces apoptosis and cell-cycle arrest in nasopharyngeal carcinoma cell lines via inhibition of STAT3 phosphorylation,” Acta Pharmacologica Sinica, vol. 33, no. 5, pp. 682–690, 2012. View at: Publisher Site | Google Scholar
  34. J. M. Mast, D. Tse, K. Shee et al., “Diarylidenylpiperidones, H-4073 and HO-3867, induce G2/M cell-cycle arrest, apoptosis and inhibit STAT3 phosphorylation in human pancreatic cancer cells,” Cell Biochemistry and Biophysics, vol. 77, no. 2, pp. 109–119, 2019. View at: Publisher Site | Google Scholar
  35. M. Subramaniam, S. K. Liew, K. Awang, N. Ahmed, and N. H. Nagoor, “Inactivation of nuclear factor & kappa;B by MIP-based drug combinations augments cell death of breast cancer cells,” Drug Design, Development and Therapy, vol. 12, pp. 1053–1063, 2018. View at: Publisher Site | Google Scholar
  36. C. In, W. Zuo, X. Wang, L. Wei, Q. Guo, and X. Song, “Lapatinib inhibits the activation of NF-κB through reducing phosphorylation of IκB-α in breast cancer cells,” Oncology Reports, vol. 29, no. 2, pp. 812–818, 2013. View at: Publisher Site | Google Scholar
  37. J. H. Park, Y. H. Seo, J. H. Jang et al., “Asiatic acid attenuates methamphetamine-induced neuroinflammation and neurotoxicity through blocking of NF-kB/STAT3/ERK and mitochondria-mediated apoptosis pathway,” Journal of Neuroinflammation, vol. 14, no. 1, pp. 240–254, 2017. View at: Publisher Site | Google Scholar
  38. Y. Yang, G. Wang, W. Wu et al., “Camalexin induces apoptosis via the ROS-ER stress-mitochondrial apoptosis pathway in AML cells,” Oxidative Medicine and Cellular Longevity, vol. 2018, Article ID 7426950, 14 pages, 2018. View at: Publisher Site | Google Scholar
  39. C.-Y. Lee, C.-H. Su, P.-K. Tsai et al., “Cadmium nitrate-induced neuronal apoptosis is protected by N-acetyl-l-cysteine via reducing reactive oxygen species generation and mitochondria dysfunction,” Biomedicine & Pharmacotherapy, vol. 108, pp. 448–456, 2018. View at: Publisher Site | Google Scholar

Copyright © 2020 Gui-Nan Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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