Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 2

Research Article

Polygonum multiflorum Thunb. Extract Stimulates Melanogenesis by Induction of COX2 Expression through the Activation of p38 MAPK in B16F10 Mouse Melanoma Cells

Figure 2

PME treatment enhanced melanin production and tyrosinase activity in B16F10 cells. B16F10 cells were treated with 2.5, 5, and 10 μg/mL PME or with 100 μM IBMX for 96 h. (a) Representative images of cell lysates and pellets of B16F10 cells after incubation. (b) Absorbance of 490 nm was measured in each cell lysate, and melanin levels were calculated relative to those of control cells. (c) Absorbance of 475 nm, indicating the L-DOPA oxidation rate, was measured in each cell lysate, and tyrosinase activity was calculated relative to that of control cells. Control (Con): vehicle only. Data are presented as mean ± SEM. , , vs. control.
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