Alleviation by Mahuang Fuzi and Shenzhuo Decoction in High Glucose-Induced Podocyte Injury by Inhibiting the Activation of Wnt/β-Catenin Signaling Pathway, Resulting in Activation of Podocyte Autophagy

Figure 4

MFSD restores podocyte autophagy affected by high glucose. (a) Immunofluorescence for the expression of LC3B, beclin1, and p62/SQSTM1 in the control group, high glucose group, and MFSD group; red represents corresponding protein, green represents cytoskeleton, and blue represents nucleus. (b) The protein expressions of LC3B, beclin1, and p62/SQSTM1 were analyzed by western blot. B1 represents control group, B2 represents high glucose group, B3 represents DKK1 group, and B4 represents MFSD group. (c) Quantitative results of western blotting analysis. The obtained values of LC3B, beclin1, and p62/SQSTM1 were normalized to the GAPDH. , , and compared with control group; , , and compared with high glucose group.

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