Protective Effect of a Mixture of Astragalus membranaceus and Lithospermum erythrorhizon Extract against Hepatic Steatosis in High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Mice

Figure 4

Effects of ALM16 and individual extracts (AM and LE) on hepatic AMPK and ACC phosphorylation in HFD-fed mice. (a) Western blot analysis of AMPK, p-AMPK, ACC, and p-ACC in the liver tissues of HFD-fed mice. Bands were quantified as the ratio of (b) p-AMPK/AMPK and (c) p-ACC/ACC, respectively, with values normalized to the HFD group (n = 4). Values are presented as mean ± standard error of the mean (SEM). ^#, ^##, and ^### vs ND group; , , and vs HFD group. ND: normal diet; HFD: high fat diet; AM: HFD + AM extract (100 mg/kg); LE: HFD + LE extract (100 mg/kg); ALM16-L: HFD + ALM16 (50 mg/kg); ALM16-M: HFD + ALM16 (100 mg/kg); ALM16-H: HFD + ALM16 (200 mg/kg); MT: HFD + milk thistle extract (100 mg/kg).

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