Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 2

Research Article

Ginsenoside Rg1 Drives Stimulations of Timosaponin AIII-Induced Anticancer Effects in Human Osteosarcoma Cells

Figure 2

Effect of Rg1 on TA3-induced apoptosis in MG63 human osteosarcoma cells. (a) Apoptosis of MG63 cells was synergistically induced by Rg1 and TA3. TA3 (6 μM) and/or Rg1 (250 μM) were applied to the cells for 24 h. Then, Annexin V/PI apoptosis assay was performed. (b) The results from Annexin V/PI apoptosis assay were quantified. (c) DNA fragmentation was synergistically induced by Rg1 and TA3. TA3 (6 μM) and/or Rg1 (250 μM) were applied to the cells for 24 h. Then, the TUNEL assay was performed. The results were analyzed statistically by ANOVA and Tukey’s test ().
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