Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 5

Research Article

Zi Shen Huo Luo Formula Prevents Aldosterone-Induced Cardiomyocyte Hypertrophy and Cardiac Fibroblast Proliferation by Regulating the Striatin-Mediated MR/EGFR/ERK Signaling Pathway

Figure 5

Functional identification of the role of striatin in the nongenomic effects of aldosterone on cardiomyocytes and cardiac fibroblasts by siRNA. In the control group, cardiomyocytes and cardiac fibroblasts were pretreated with blank serum, the cardiomyocytes were then incubated with 10−9 mol/l aldosterone for 30 min, and the cardiac fibroblasts were incubated with 10−7 mol/l aldosterone for 30 min. Cells in the siRNA STR group were transfected with siRNA STR, pretreated with blank serum for 2 h, and incubated with aldosterone. Cells in the siRNA + ZSHLF group were transfected with siRNA STR, pretreated with ZSHLF-containing serum for 2 h, and then incubated with aldosterone. (a) Cells subjected to immunofluorescent assays using DAPI (blue) and FITC (green). (b) Cardiomyocyte surface area (relative to the control group) determined by α-actinin immunofluorescence staining. (c) OD values of cardiac fibroblasts detected by the CCK-8 assay. (d) Hydroxyproline content in the cardiac fibroblast culture supernatant. vs. the control group; vs. the siRNA STR group.

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