Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer
CM-EE induced apoptotic cell death in human and mouse breast cancer cells. The cells were treated with CM-EE or CHX (cycloheximide: 2 ng/ml) + TNFα (tumor necrosis factor-α: 10 µg/ml) for 72 h and then stained with APC-Annexin V and 7-AAD (a) or western blotting was conducted with PARP, caspase 3/7 antibodies (b). (a) Representative dot plot for Annexin V and 7-AAD staining and apoptotic cell death of human and mouse breast cancer cells. The results are presented as mean ± standard deviation (SD) for three independent experiments. Unpaired t test was used for statistical analysis. ,, and compared with control. (b) Western blot image and analysis of protein levels for total and cleaved PARP, caspase 3/7.
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