Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer

Figure 3

CM-EE elicits the apoptotic cell surface exposure of CRT, HSP70, and HSP90 on human and mouse breast cancer cells. (a) Human breast cancer cells (MDA-MB-231 cells) and (c) mouse breast cancer cells (4T1-neu-HA cells) were treated with dose-dependent concentrations of CM-EE (0–100 µg/ml) for 72 h and then stained with anti-CRT, anti-HSP70, and anti-HSP90 and analyzed by flow cytometry. (b) MDA-MB-231 cells and (d) 4T1-neu-HA cells were treated with 100 µg/ml CM-EE for 1, 3, 6, 12, 24, 48, and 72 h and then stained with anti-CRT and analyzed by flow cytometry. The results are presented as mean ± standard deviation (SD) for triple replicates. The unpaired t test was used for statistical analysis. , , and compared with control.

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