Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems

Table 3

Hematological parameters of male and female SD rats in the 13-week repeated oral toxicity study of EAG.

Tests	EAG (mg/kg/day)
	0	1,250	2,500	5,000
Male
RBC (10^6/μL)	8.99 ± 0.55	9.01 ± 0.22^a	8.81 ± 0.37	8.87 ± 0.42
HGB (g/dL)	15.3 ± 0.5	15.5 ± 0.4^a	15.0 ± 0.5	15.0 ± 0.4
HCT (%)	47.3 ± 1.8	47.9 ± 1.4^a	46.7 ± 1.3	46.6 ± 1.6
MCV (fL)	52.7 ± 1.9	53.2 ± 1.0^a	53.1 ± 2.6	52.5 ± 1.1
MCH (pg)	17.1 ± 0.8	17.2 ± 0.3^a	17.0 ± 1.0	16.9 ± 0.5
MCHC (g/dL)	32.4 ± 0.5	32.3 ± 0.4^a	32.1 ± 0.8	32.3 ± 0.5
PLT (10^3/μL)	919.2 ± 61.3	905.9 ± 93.0^a	890.4 ± 71.7	933.3 ± 74.8
WBC (10^3/μL)	6.30 ± 1.37	7.22 ± 2.18^a	7.54 ± 1.16	7.91 ± 0.97
NEU (10^3/μL)	1.3 ± 0.3	1.5 ± 0.6^a	1.6 ± 0.7	1.1 ± 0.2
LYM (10^3/μL)	4.6 ± 1.2	5.2 ± 1.6^a	5.4 ± 1.0	6.3 ± 1.0^∗
MONO (10^3/μL)	0.28 ± 0.12	0.31 ± 0.10^a	0.32 ± 0.11	0.30 ± 0.06
EOS (10^3/μL)	0.11 ± 0.04	0.11 ± 0.03^a	0.13 ± 0.02	0.10 ± 0.03
BASO (10^3/μL)	0.01 ± 0.01	0.01 ± 0.01^a	0.01 ± 0.00	0.01 ± 0.00
PT (sec)	8.0 ± 0.2	8.1 ± 0.2^a	8.0 ± 0.2	7.8 ± 0.2
Female
RBC (10^6/μL)	7.98 ± 0.35	7.72 ± 0.30	7.86 ± 0.22	7.94 ± 0.28
HGB (g/dL)	14.3 ± 0.3	14.0 ± 0.4	14.1 ± 0.3	14.3 ± 0.4
HCT (%)	43.5 ± 1.3	42.8 ± 1.2	43.2 ± 1.9	43.7 ± 1.2
MCV (fL)	54.6 ± 1.8	55.5 ± 2.1	54.9 ± 0.8	55.0 ± 0.8
MCH (pg)	17.9 ± 0.6	18.1 ± 0.7	18.0 ± 0.4	17.9 ± 0.3
MCHC (g/dL)	32.8 ± 0.2	32.7 ± 0.4	32.7 ± 0.4	32.6 ± 0.4
PLT (10^3/μL)	969.9 ± 60.9	1023.9 ± 89.3	977.4 ± 87.8	950.3 ± 66.4
WBC (10^3/μL)	3.67 ± 0.95	3.75 ± 1.03	3.84 ± 1.22	4.01 ± 1.18
NEU (10^3/μL)	0.5 ± 0.1	0.5 ± 0.1	0.5 ± 0.2	0.5 ± 0.2
LYM (10^3/μL)	3.0 ± 0.9	3.0 ± 0.9	3.1 ± 1.0	3.3 ± 0.9
MONO (10^3/μL)	0.09 ± 0.04	0.11 ± 0.03	0.11 ± 0.04	0.13 ± 0.05
EOS (10^3/μL)	0.08 ± 0.03	0.08 ± 0.02	0.08 ± 0.03	0.07 ± 0.03
BASO (10^3/μL)	0.01 ± 0.01	0.00 ± 0.01	0.00 ± 0.00	0.00 ± 0.01
PT (sec)	7.7 ± 0.2	7.4 ± 0.2^##	7.3 ± 0.2^##	7.4 ± 0.2^##

Data are expressed as mean ± standard deviation.Significant difference at levels compared with the negative control by Scheffe multiple range test. ^##Significant difference at levels compared with the negative control by Duncan multiple range test. ^aNumber of animals in the group was 9; otherwise mean of 10 animals/sex/group. RBC, red blood cell; HGB, hemoglobin concentration; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; PLT, platelet count; WBC, white blood cell; NEU, neutrophil; LYM, lymphocyte; MONO, monocyte; EOS, eosinophil; BASO, basophil; and PT, prothrombin time.