Hypoxia Upregulates NOTCH3 Signaling Pathway to Promote Endothelial-Mesenchymal Transition in Pulmonary Artery Endothelial Cells

Figure 3

Blocking NOTCH3 (DAPT treatment (NOTCH3-specific inhibitor)) inhibits hypoxia-induced endothelial cell EnMT. (a) Observation of cell morphology. (b) Western blotting analysis of VE-cadherin expression. (c) Western blotting analysis of Vimentin expression. (d) Cell viability experiment on cell proliferation by MTT. (e) Transwell assay to evaluate cell migration. (f) Scratch test to evaluate cell migration. (g) The fluorescent microscope of immunofluorescence staining shows the intensity of α-SMA antibody staining. DAPI was used for nuclear staining. (h) The fluorescent microscope of immunofluorescence staining shows the intensity of vWF expression. DAPI was used for nuclear staining. , Student's t-test. All data are presented as the mean ± SD for biological triplicate experiments.