Tert-Butylhydroquinone Prevents Oxidative Stress-Mediated Apoptosis and Extracellular Matrix Degradation in Rat Chondrocytes

Figure 1

TBHQ prevented excessive generation of ROS, increased the levels of SOD, and decreased the levels of MDA, and promoted cell apoptosis in rat chondrocytes treated with 20 μM TBHP. (a) The structural formula of TBHQ. (b-c) Chondrocytes stained with DCFH-DA and then quantified through flow cytometry to determine the levels of ROS. The results showed that 2.5 and 5 μM of TBHQ effectively prevented excessive generation of ROS in rat chondrocytes treated with 20 μM THBP. (d-e) Annexin V/PI staining was used to determine the apoptosis of rat chondrocytes. Flow cytometry analysis showed that treatment with 2.5 and 5 μM  of TBHQ significantly reduced the rate of apoptosis in chondrocytes treated with 20 μM  TBHP. (f-g) 2.5 and 5 μM  of TBHQ significantly decreased the levels of MDA and increased the levels of SOD in rat chondrocytes treated with 20 μM THBP. and .

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