Effects of Nigella sativa on Performance, Blood Profiles, and Antibody Titer against Newcastle Disease in Broilers
Table 5
Effects of N. sativa on biochemical parameters (Mean ± SEM) of broilers.
Age (day)
Group
Alb (g/dL)
TP (g/dL)
Glu (mg/dL)
Cal (mg/dL)
Phos (mg/dL)
Day1
T0–T7
0.62 ± 0.03NS
1.73 ± 0.10NS
276 ± 7.5NS
8.19 ± 0.07NS
2.09 ± 0.02NS
Day 7
T0
0.74 ± 0.05a
1.85 ± 0.10a
220.6 ± 304ad
8.0 ± 0.03a
3.03 ± 0.04a
T1
0.72 ± 0.02a
1.86 ± 0.02a
216.0 ± 0.94a
8.06 ± 0.05a
30.8 ± 0.12a
T2
0.76 ± 0.02a
1.87 ± 0.03ab
225.0 ± 2.56abd
8.26 ± 0.04a
3.52 ± 0.06b
T3
0.78 ± 0.03a
2.06 ± 0.06b
232.8 ± 1.99bc
8.36 ± 0.11b
3.66 ± 0.08bc
T4
0.84 ± 0.03a
2.06 ± 0.10b
240.4 ± 0.52b
9.60 ± 0.05c
3.78 ± 0.08c
T5
0.80 ± 0.05a
1.96 ± 0.06 b
230.4 ± 2.88cd
8.18 ± 0.03ab
3.74 ± 0.04cb
T6
0.70 ± 0.01a
1.78 ± 0.05ab
227.0 ± 1.86db
7.52 ± 0.04ad
3.71 ± 0.09cb
T7
0.68 ± 0.03a
1.74 ± 0.08a
221.4 ± 2.04ad
7.25 ± 0.05d
3.61 ± 0.05cb
Day 14
T0
0.74 ± 0.07a
2.14 ± 0.05a
221.8 ± 1.93ad
8.50 ± 0.14a
3.26 ± 0.08a
T1
0.68 ± 0.07a
1.92 ± 0.08abc
217.6 ± 2.18a
8.48 ± 0.16a
3.14 ± 0.03a
T2
0.70 ± 0.05a
1.96 ± 0.15abc
239.4 ± 1.24b
8.90 ± 0.06a
3.40 ± 0.06b
T3
0.72 ± 0.05a
2.04 ± 0.12abc
246.8 ± 3.39bc
9.06 ± 0.04a
3.48 ± 0.05b
T4
0.80 ± 0.0a
2.26 ± 0.14a
254.6 ± 1.03c
9.06 ± 0.08b
3.86 ± 0.07c
T5
0.70 ± 0.07a
1.90 ± 0.14abc
250.0 ± 3.56c
7.44 ± 0.12c
3.50 ± 0.06b
T6
0.68 ± 0.06a
1.86 ± 0.08ab
244.2 ± 3.42bc
7.24 ± 0.16c
3.02 ± 0.05a
T7
0.64 ± 0.04a
1.68 ± 0.13cb
227.4 ± 3.50d
6.94 ± 0.13c
3.10 ± 0.04a
Day 21
T0
0.94 ± 0.07a
2.34 ± 0.08a
220.8 ± 1.9ad
8.24 ± 0.16a
3.30 ± 0.07a
T1
0.72 ± 0.07a
2.08 ± 0.10abc
224.2 ± 2.1ad
8.18 ± 0.16b
3.20 ± 0.06a
T2
0.94 ± 0.05a
2.12 ± 0.17abc
251.2 ± 1.2b
9.50 ± 0.06b
3.66 ± 0.03 b
T3
0.98 ± 0.05a
2.22 ± 0.12abc
269.0 ± 2.3c
9.66 ± 0.04b
3.80 ± 0.02c
T4
1.0 ± 0.05a
2.46 ± 0.16a
276.6 ± 1.0c
10.98 ± 0.08c
3.92 ± 0.03c
T5
0.90 ± 0.07a
2.06 ± 0.15abc
272.0 ± 2.5c
7.96 ± 0.14a
3.62 ± 0.05b
T6
0.88 ± 0.06a
1.98 ± 0.10 b
269.2 ± 2.4c
7.76 ± 0.16a
3.14 ± 0.08ad
T7
0.88 ± 0.04a
1.80 ± 0.15cb
224.4 ± 3.5d
7.47 ± 0.13a
3.00 ± 0.05 d
Day 28
T0
1.34 ± 0.07a
2.53 ± 0.10a
209.8 ± 1.9a
8.44 ± 0.16a
3.38 ± 0.05a
T1
1.34 ± 0.07a
2.56 ± 0.10a
213.6 ± 2.1a
8.36 ± 0.16a
3.30 ± 0.08a
T2
1.60 ± 0.05b
3.08 ± 0.10bcd
260.2 ± 1.2b
9.80 ± 0.08b
3.70 ± 0.03b
T3
1.62 ± 0.05b
3.18 ± 0.09bcd
278.0 ± 2.3c
9.96 ± 0.04b
3.90 ± 0.02c
T4
1.80 ± 0.05c
3.40 ± 0.10b
285.6 ± 1.0c
11.08 ± 0.08c
4.02 ± 0.03c
T5
1.68 ± 0.07bc
3.18 ± 0.10bcd
281.0 ± 2.6c
8.26 ± 0.14ad
3.72 ± 0.05b
T6
1.68 ± 0.06bc
3.04 ± 0.10cd
278.2 ± 2.4c
8.06 ± 0.16ad
3.24 ± 0.08ad
T7
1.58 ± 0.04b
3.00 ± 0.08dc
228.0 ± 2.5d
7.76 ± 0.13d
3.10 ± 0.05d
Day 35
T0
1.54 ± 0.07a
2.94 ± 0.12a
203.8 ± 1.93a
8.54 ± 0.16a
3.40 ± 0.07a
T1
1.54 ± 0.07a
2.94 ± 0.12a
211.6 ± 2.18a
8.68 ± 0.16a
3.32 ± 0.06a
T2
1.70 ± 0.05ab
3.24 ± 0.11abc
255.2 ± 1.24b
9.90 ± 0.06bc
3.76 ± 0.03b
T3
1.82 ± 0.06b
3.36 ± 0.11bc
273.0 ± 3.39c
10.05 ± 0.04bc
4.00 ± 0.02c
T4
1.88 ± 0.05b
3.56 ± 0.10b
280.6 ± 1.02c
10.38 ± 0.08c
4.04 ± 0.04c
T5
1.78 ± 0.07bc
3.44 ± 0.12bc
272.0 ± 3.56c
8.36 ± 0.14a
3.74 ± 0.05b
T6
1.72 ± 0.06ab
3.22 ± 0.11abc
259.2 ± 3.42db
8.16 ± 0.16ad
3.26 ± 0.05ad
T7
1.68 ± 0.06ac
3.18 ± 0.11ca
204.4 ± 3.50ea
7.80 ± 0.14d
3.14 ± 0.06d
Day 42
T0
1.74 ± 0.07a
3.24 ± 0.12a
180.8 ± 1.92a
8.92 ± 0.1a
3.60 ± 0.08a
T1
1.72 ± 0.07a
3.22 ± 0.12a
194.6 ± 2.17b
9.14 ± 0.2a
3.52 ± 0.06a
T2
1.92 ± 0.06abc
3.54 ± 0.11abc
232.2 ± 3.38c
11.68 ± 0.1b
3.96 ± 0.04b
T3
2.02 ± 0.05bc
3.66 ± 0.10bc
250.0 ± 1.12d
11.86 ± 0.1b
4.20 ± 0.03c
T4
2.08 ± 0.06 b
3.86 ± 0.12b
257.6 ± 3.56d
12.06 ± 0.1b
4.24 ± 0.04c
T5
1.98 ± 0.07bc
3.74 ± 0.11bc
243.6 ± 3.42d
9.92 ± 0.2c
3.94 ± 0.05 b
T6
1.90 ± 0.06abc
3.52 ± 0.11abc
238.2 ± 2.50c
9.72 ± 0.1c
3.46 ± 0.06a
T7
1.85 ± 0.05ac
3.48 ± 0.11ac
198.0 ± 1.12b
9.36 ± 0.1da
3.34 ± 0.06da
Note. Alb: albumin, TP: total protein, Glu: glucose, Cal: calcium, and Ph: phosphorous. T0 = control 1 (without vaccination and without supplementation of N. sativa in diet), T1 = control 2 (T0 + vaccination), T2 = T1 + N. sativa 0.5%, T3 = T1 + N. sativa 1%, T4 = T1 + N. sativa 2%, T5 = T1 + N. sativa 4%, T6 = T1 + N. sativa 8%, and T7 = T1 + N. sativa 16%. Different superscript letters in each column/week indicate significant () differences between groups, and NS stands for not significant ().
