Inhibitory Effects of Inonotus obliquus Polysaccharide on Inflammatory Response in Toxoplasma gondii-Infected RAW264.7 Macrophages

Figure 4

Regulation of IOP on NF-κB (a) and (b) and MAPKs (c) and (d) signaling pathways in RAW264.7 macrophages infected with T. gondii. (a, c) RAW264.7 macrophages were infected with T. gondii and treated with IOP or SD; the phosphorylated levels of IKKα/β, IκBα, NF-κB p65, p38, JNK, and ERK1/2 were determined by Western blot analysis. Values are expressed as means ± SD of three independent experiments. vs. normal group; , vs. model group. (b, d) RAW264.7 macrophages were infected with T. gondii and treated with IOP or SD; NF-κB p65 and c-Jun activation was observed by immunocytochemistry staining. Blue represents nucleus; green represents NF-κB p65 and c-Jun. The image magnification is 400×.

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