Antimicrobial and Immunomodulatory Activity of Herb Extracts Used in Burn Wound Healing: “San Huang Powder”

Figure 6

The in vitro assay of the anti-inflammatory activity of herb materials used in this study. (a) HMEC-1 cells were treated with 0.04, 0.12, 0.36, and 1.08 mg/mL herb, including Rhubarb, Scutellaria root, Phellodendron bark, and Coptidis rhizome for 24 h. Cell numbers were calculated and shown as mean ± SD. The IC₅₀ (half maximal inhibitory concentration) values of Rhubarb, Scutellaria root, Phellodendron bark, and Coptidis rhizome for HMEC-1 cell were 0.88 ± 0.05, 0.71 ± 0.06, 2.65 ± 0.59, and 0.44 ± 0.05 mg/mL, respectively. The 0 group is without herb treatment, only DMSO solvent as control. The cell survival rate of control was taken as 100%. (b) HMEC-1 cells were treated with 0.1% DMSO, 0.8 mg/mL Rhubarb, 0.7 mg/mL Scutellaria root, 2.6 mg/mL Phellodendron bark, or 0.4 mg/mL Coptidis rhizome for 1 h and then untreated (DMSO and LPS groups) or treated with 0.2 g/mL LPS for additional 24 h. Relative folds of OD450 nm values were calculated and shown as mean ± SD, taking the value of DMSO group as 1.0. Through one-tailed test analysis, denotes statistical significance () compared with DMSO and represents two reproducible results.

(a)

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