Research Article

Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity

Figure 3

Effects of INH/RIF on the mRNA level of ALAS1 and FOXO1 in L-02 cells and HepG2 cells. (a) L-02 cells were treated without the drug as the blank control, with INH (600 μM), RIF (200 μM), or INH/RIF (600/200 μM) for 24 h. ALAS1 protein expression levels were quantified by western blotting. (b) HepG2 cells were treated without the drug, with INH (600 μM), RIF (200 μM), or INH/RIF (600/200 μM) for 24 h. ALAS1 protein expression levels were quantified by western blotting. (c) L-02 cells were treated with the same concentration of INH and/or RIF for 24 h. FOXO1 protein expression levels were quantified by western blotting. (d) HepG2 cells were treated with the same concentration of INH and/or RIF for 24 h. FOXO1 protein expression levels were quantified by western blotting. (e) L-02 cells were treated with the same concentration of INH and/or RIF for 24 h. Relative protein (in the nucleus) levels of FOXO1 were quantified by western blotting. (f) HepG2 cells were treated with the same concentration of INH and/or RIF for 24 h. Relative protein (in the nucleus) levels of FOXO1 were quantified by western blotting.
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