Research Article

Genetic and Functional Evaluation of the Role of FOXO1 in Antituberculosis Drug-Induced Hepatotoxicity

Figure 4

Effect of FOXO1 on cell survival rate and ALAS transcription level. (a) HepG2 cells were transiently transfected with siRNAs of FOXO1 (shF1 and shF3) or siRNA control. “−” = without coadministration of INH/RIF (600/200 μM); “+” = with coadministration of INH/RIF (600/200 μM) for 24 h. (b) HepG2 cells were transiently transfected with siRNAs of FOXO1 (shF1 and shF3) or siRNA control for 24 h. ALAS1 mRNA expression levels were quantified by RT-qPCR. (c) HepG2 cells were transiently transfected with siRNAs of FOXO1 (shF1 and shF3) or siRNA control for 24 h. ALAS1 protein expression levels were quantified by western blotting.
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