Saireito Improves Lymphatic Function and Prevents UVB-Induced Acute Inflammation and Photodamage in HR-1 Hairless Mice

Figure 4

Effect of SRT on UVB-induced inflammatory cell infiltration and proinflammatory cytokine production. (a) Skin sections were collected at 48 h after irradiation, and immunohistochemical staining was performed for Iba-1. Scale bar = 200 μm. (b) SRT significantly inhibited UVB-induced infiltration of macrophages at 48 h and 72 h. (c) Skin sections were collected at 24 h after irradiation, and immunohistochemical staining was performed for Ly-6G/6C. Arrows indicate Ly-6G/6C-positive cells in the dermis. Scale bar = 100 μm. (d) SRT significantly inhibited neutrophil infiltration at 24 h after irradiation. (e) and (f) SRT significantly inhibited UVB-induced increase in IL-1β and IL-6 at 24 h after irradiation. On the contrary, SRT increased the expression level of IL-1β and IL-6 at 72 h compared with the vehicle group. Data were expressed as mean ± SEM (n = 4-5). and vs. control, Dunnett’s test. and vs. vehicle, Student’s t-test. SRT: Saireito extract; UVB: ultraviolet B.

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