Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway

Figure 6

ZnS inhibits the AKR1C1-mediated JAK2/STAT3 pathway. (a) The relative protein expression of AKR1C1, JAK2, p-JAK2, STAT3, and p-STAT3 in Huh7 and SMMC7721 cells examined by Western blot. Cells were treated with 0.5 µM ZnS for Huh7 and 1.0 µM for SMMC7721 cells and/or 10 mM 3-methyladenine (3-MA) for 48 h.  < 0.01 and  < 0.001 vs. the control group.  < 0.05 and  < 0.001 vs. the ZnS + 3-MA group. (b) The relative protein levels of AKR1C1, JAK2, p-JAK2, STAT3, and p-STAT3 in Huh7 and SMMC7721 cells measured by Western blot. Huh7 and SMMC7721 cells treated with ZnS and/or lenti-NC/lenti-AKR1C1.  < 0.01 vs. the control group.  < 0.05,  < 0.01, and  < 0.001 vs. the ZnS + lenti-NC group.

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(b)