Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

Figure 3

Effect of HPAC on NF-κB nuclear translocation. RAW 264.7 cells were pretreated with the indicated concentrations of HPAC for 2 h followed by LPS for 2 h. (a) Nuclear and cytosolic proteins were isolated and analyzed by western blotting using anti-NF-κB p65 antibody. Lamin A/C, HSP 90, and β-actin were used as internal controls for the nuclear and cytosolic fractions, respectively. (b) Quantitative analysis of nuclear NF-κB p65 expression relative to Lamin A/C. (c) Western blot analysis of p-IκBα, IκBα, and β-actin. (d) Quantitative analysis of p-IκBα expression relative to IκBα. DXM was used as a positive control. The data are presented as means ± SEM (n = 3). versus control group, and versus LPS group.

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