Mogroside V Alleviates Lipopolysaccharide-Induced Neuroinflammation via Inhibition of TLR4-MyD88 and Activation of AKT/AMPK-Nrf2 Signaling Pathway

Figure 5

Mog suppressed the TLR4-mediated NF-κB and MAPKs signaling pathways in LPS-treated BV-2 cells. (a) Cells were cultured with Mog (0, 6.25, 12.5, and 25 μM) for 2 h and then treated with LPS (1 μg/mL) for another 1 h. The proteins expressions of TLR4, MyD88, p-ERK, ERK, p-JNK, JNK, p-P38, P38, p-IκBα, IκBα, p-P65, and P65 were measured by western blot analysis. (b–i) Quantification of relative protein expression was performed by densitometric analysis. Similar results were obtained from three independent experiments. The values presented are the means ± SEM.  < 0.05 and  < 0.01 versus control group; ^# < 0.05 and ^## < 0.01 versus LPS group.

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