Kai-Xin-San Attenuates Doxorubicin-Induced Cognitive Impairment by Reducing Inflammation, Oxidative Stress, and Neural Degeneration in 4T1 Breast Cancer Mice

Figure 7

IBA-1 immunostaining in the mice hippocampus. Merged overview of the relevant hippocampus region with IBA-1 in red and nuclear counterstaining with DAPI in blue (magnification 400x). The white arrow depicts the IBA-1⁺ cell shown in the enlarged image. NC group: very mild level of IBA-1 (a); TC group: moderate level of IBA-1 (b); TC + KXS group: very mild level of IBA-1 (c); DOX group: severe level of IBA-1 (d); DOX + KXS group: IBA-1 expressions at a moderate level of IBA-1 (e). IH, scale bar = 50 μm, n = 3/per group. IBA-1: ionized calcium-binding adapter molecule; DAPI: 4′,6-diamidino-2-phenylindole; DG: dentate gyrus. The process length of IBA-1⁺ cells (f) (i), the endpoint of IBA-1⁺ cells in the DG (f) (ii). mean ± SEM, , , and compared to the DOX group.

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