Duchesnea indica Extract Attenuates Coal Fly Ash-Induced Inflammation in Murine Alveolar Macrophages through the NF-Kappa B Pathway

Figure 5

Effect of the DIE on CFA-induced NF-κB phosphorylation in MH-S cells. (a) After 18 h of incubation with CFA (2.5 µg/mL), the protein levels of iNOS, COX-2, p-IκB, and p-NFκB were determined using western blot. β-Actin was used as a loading control. (b–e) Densitometric analysis of protein expression levels which were measured using the ImageJ software. The cells were seeded in a six-well plate, and the doses of DIE were used as 12.5, 25, 50, and 100 μg/mL. All values were expressed as standard error of mean ± (SEM) from three independent experiments. , , and when compared with the only CFA-treated group.

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