Tanshinone IIA Inhibits Osteosarcoma Growth through a Src Kinase-Dependent Mechanism

Figure 8

Tanshinone IIA can inhibit the growth of osteosarcoma in tumor-bearing nude mice. (a) Tumor growth curve of tumor-bearing nude mice after inoculation. (b) X-rays taken before and after tanshinone IIA treatment showed that the soft tissue shadow of the left hind limb of the experimental group was reduced compared with that of the control group. (c) Tumor growth curve showed that tanshinone IIA inhibited the tumor growth. (d) Weight measurements of the tumor following tanshinone IIA treatment. (e) Tanshinone IIA increased tumor cell differentiation and light cell nucleus measured by HE staining (×200). (f) Tanshinone IIA inhibited expression of Src, p-Src, p-MAPK, p-ERK1/2, p-PI3K, and p-AKt in osteosarcoma tissue measured by Western blot (; pretreated, the mice were not treated with tanshinone IIA or saline; treated, the mice were treated with tanshinone IIA or saline).

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