Aboriginal Bacterial Flora in the Uricase-Deficient Rat Gut is Not the Main Factor Affecting Serum Uric Acid

Figure 5

Bacterial abundance in Uox^-/- rat stool measured by DNA (mean + SD, n = 6). (a) Total DNA extracted from rat stool (nos. 1–6 were from WT rat stool, nos. 7–12 were from normal Uox^-/- rats’, nos. 13–18 were from Uox^-/- rats treated with the combination of antibiotics, and “M” was the marker with the brightest band of 5,000 bp); (b) DNA content in samples (mean + SD, n = 6); (c) the correlation between the amount of bacterial DNA and the quantity of living bacteria (n = 18), , Pearson’s correlation (two-tailed); (d) cycle threshold (CT) values of 16S rDNA in samples (mean + SD, n = 6); (e) copies of 16S rDAN in samples (mean + SD, n = 6); (f) the correlation between CT values and the quantity of living bacteria (n = 18), , Pearson’s correlation (two-tailed). WT, stool from the wild-type rats; Uox^-/--1, stool from the normal Uox^-/- rats; Uox^-/-−2, stool from the Uox^-/- rats treated with ampicillin (90 mg/kg) and ciprofloxacin (150 mg/kg) for 5 days. vs. WT; vs. Uox^-/-−1, one-way ANOVA.

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