Chemical Analysis of the Ingredients of 20% Aqueous Ethanol Extract of Nardostachys jatamansi through Phytochemical Study and Evaluation of Anti-Neuroinflammatory Component

Figure 6

Effects of NJ20 on LPS-induced production of nitrite and PGE₂, and the protein expression of iNOS and COX-2 in primary microglial cells. Cells were pre-treated with or without the indicated concentration of NJ20 for 3 h and stimulated with LPS (1 μg/mL) for 24 h. ((a), (b)) The collected supernatants were used to determine nitrite and PGE₂ levels using the Griess reaction and appropriate ELISA kits. Values are presented as mean ± SD of three independent experiments. (c) The collected cells were lysed, and the lysates were then used to determine the expression of iNOS and COX-2 proteins by western blot analysis. Representative blots from three independent experiments are shown. compared with the control group. , , and compared with the LPS group.

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