Research Article

Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine Secretion

Figure 5

Paeoniflorin promoted TGR5/TRPA1 signaling activation in EC cells. RIN-14B cells were treated with different concentrations of PAE (20, 40, or 80 μg/ml) for 24 hours. (a) The expression of TGR5 was detected by western blot. (b, c) The expression of TGR5 was tested by IF stain. Scale 50 μm. (d) The expression of TRPA1, PLC-γ1, and PIP2 in RIN-14B cells was detected by western blot. Data are mean ± SEM. vs. Con group.
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