Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Figure 2

Effects of yixintongmai on migration and MMP-9 expression of VSMCs cultured with high glucose. NG: normal glucose (5.5 mM); HG: high glucose (25 mM); HG + Y: high glucose (25 mM) and yixintongmai powder (360 μg/ml); MG: normal glucose (5.5 mM) and mannitol (19.5 mM). The migration of VSMCs was assessed using transwell assay (a, b) and scratch wound assay (c, d). In the transwell assay, the staining was examined by fluorescence microscopy at 100x magnification in three random fields. Fluorescent image (a); the mean numbers of VSMCs in three random fields (b); in wound healing assay, VSMCs migration was shown at 0 h and 24 h (c) and migration rate (d); Western blot of MMP-9 expression (e) was quantified by densitometry using ImageJ V1.8.0 (f). Densitometry quantification values are shown by fold over NG or HG. Values are expressed as means ± SD of triplicate experiments. ; .

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