Genetic Toxicology and Safety Pharmacological Evaluation of Forsythin

Table 1

Results of the bacterial reverse mutation test (Ames test) for forsythin.

S9	Chemical	Dose (μg/plate)	His^+ revertant colonies/plate
			TA97	TA98	TA100	TA102	TA1535
−	DMSO (1%)^a	0	137.4 ± 30.8	16.4 ± 2.5	75.2 ± 43.5	263.8 ± 127.4	12.8 ± 0.5
	Dexon^b	100	1609.0 ± 273.9	1122.2 ± 593.0	1429.6 ± 101.3	2253.1 ± 431.8
	Sodium azide^b	15					2170.2 ± 332.2
	Forsythin	2500	125.2 ± 54.2	21.0 ± 5.6	65.7 ± 51.8	333.8 ± 112.4	10.5 ± 4.4
		1250	154.0 ± 33.0	17.5 ± 4.5	53.1 ± 38.2	285.8 ± 52.8	11.8 ± 2.1
		625	128.9 ± 52.4	13.3 ± 4.7	61.4 ± 48.3	237.7 ± 118.8	11.6 ± 3.2
		312	144.8 ± 51.1	18.4 ± 9.3	61.3 ± 44.3	216.6 ± 85.3	10.2 ± 1.6
		156	135.2 ± 16.9	18.7 ± 6.0	56.3 ± 44.5	200.5 ± 42.0	11.7 ± 1.4
+	DMSO (1%)^a	0	157.1 ± 54.7	15.7 ± 4.2	77.9 ± 57.4	318.6 ± 86.6	16.7 ± 5.0
	2-Aminofluorine^b	30	1027.5 ± 296.2	2936.6 ± 686.4	768.7 ± 88.3
	1, 8-Dioxyanthraquinone^b	50				934.6 ± 69.6
	Cyclophosphamide^b	200					459.8 ± 178.9
	Forsythin	2500	160.4 ± 28.0	17.4 ± 6.0	79.8 ± 71.5	343.1 ± 172.3	11.6 ± 3.8
		1250	162.0 ± 39.6	21.8 ± 2.0	68.8 ± 54.0	344.9 ± 75.4	13.2 ± 0.9
		625	160.5 ± 38.3	17.5 ± 6.5	77.1 ± 72.9	274.1 ± 101.7	10.6 ± 1.9
		312	153.1 ± 42.1	19.3 ± 3.8	59.1 ± 64.0	307.9 ± 154.7	10.6 ± 4.0
		156	170.3 ± 41.3	17.4 ± 3.1	68.4 ± 56.2	278.8 ± 66.5	14.2 ± 3.4

Data are expressed as means ± standard deviation (SD). , compared with the negative control group; , compared with the negative control group. ^aNegative control. ^bPositive control.