Research Article

MiR-20a-5p Regulates MPP+-Induced Oxidative Stress and Neuroinflammation in HT22 Cells by Targeting IRF9/NF-κB Axis

Figure 1

MiR-20a-5p level was decreased in MPP+-treated HT22 cells. (1) The cell viability was determined using CCK-8-solution for the cells treated by different concentration of MPP+ (0.25, 0.5, 1.0, and 2.5 mM). (b and c) The level of miR-20a-5p was tested by RT-qPCR assay.  < 0.05 vs. control,  < 0.01 vs. control, and  < 0.001 vs. control. (d) Sequence alignment of miR-20a-5p and the 3’-UTR of IRF9. (e) MiR-20a-5p mimic suppressed the expression of 3’-UTR-luciferase reporter of IRF9 in 293T cells, but the mutant vector was immune to miR-20a-5p. (f) The protein level of IRF9 was tested by Western blot analysis. β-actin is a loading control.  < 0.001 vs. NC mimic. Data are expressed as mean ± SD. The experiments were repeated six times.
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