Bu-Zhong-Yi-Qi Granule Enhances Colonic Tight Junction Integrity via TLR4/NF-κB/MLCK Signaling Pathway in Ulcerative Colitis Rats

Table 2

Primers used for RT-PCR.

Target gene	Primer sequence (5’-3’)
β-Actin	Forward: 5´-AGAGGGAAATCGTGCGTGAC-3´
	Reverse: 5´-CCATACCCAGGAAGGAAGGCT-3´
TLR4	Forward: 5´-CCAGAGCCGTTGGTGTATCT-3´
	Reverse: 5´-TCAAGGCTTTTCCATCCAAC-3´
MLCK	Forward: 5´-GACGTGTTCACCCTGGTTCT-3´
	Reverse: 5´-TTTGTGCAGCATCAGTGACA-3´
NF-κb	Forward: 5´-GGCAGCACTCCTTATCAA-3´
	Reverse: 5´-GGTG TCGTCCCATCGTAG-3´
TNF-α	Forward: 5´-AGAACTCCAGGCGGTGTCT-3´
	Reverse: 5´-TCCCTCAGGGGTGTCCTTAG-3´
IL-1β	Forward: 5´-ATAGCAGCTTTCGACAGTGAG-3´
	Reverse: 5´-GTCAACTATGTCCCGACCATT-3´
IL-10	Forward: 5´-GCAGGACTTTAAGGGTTACTTGG-3´
	Reverse: 5´-TCATTCTTCACCTGCTCCACTG-3´