Aqueous Mulberry Leaf Extract Ameliorates Alcoholic Liver Injury Associating with Upregulation of Ethanol Metabolism and Suppression of Hepatic Lipogenesis

Figure 6

AME supplementation downregulated expressions of important mediators involved in fatty acid and cholesterol synthesis in the liver. Mice were randomly divided into five groups (n = 8 for each group), namely, the control group (normal diet), ethanol group (ethanol diet, the positive control), E + 0.5% AME group (ethanol diet containing 0.5% AME), E + 1% AME group (ethanol diet containing 1% AME), and E + 2% AME group (ethanol diet containing 2% AME). After 8-week administration, mice were fasted and sacrificed for the collection of hepatic tissues for protein extraction and western blot analysis. Quantitative analysis of chemiluminescence signals from three repeated western blot analyses is presented as means ± SD. ^#,  < 0.05 compared to the control group; and ,  < 0.05 and  < 0.01 compared to the ethanol group, respectively.

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