Phytochemistry, Toxicology, and Pharmacological Properties of Origanum elongatum

Table 2

Antibacterial effects of O. elongatum.

Use part	Extracts	Used method	Tested strains	Key results	References
Leaves	Ethanolic extract	Method of diffusion in solid medium; Macromethod of dilution in liquid medium	P	—	[1]
			P3	Ф = 10 ± 0.8 mm
			P65	Ф = 11 ± 0.8 mm
			P381	Ф = 8.5 ± 0.4 mm
			P2	Ф = 9 ± 0.8 mm
			P5	Ф = 8.33 ± 0.8 mm
Leaves	Aqueous extract		P	Ф = 11.66 ± 0.47 mm
			P3	Ф = 11 ± 0.95 mm
			P65	Ф = 11.83 ± 0.23 mm
			P381	Ф = 9.33 ± 1.24 mm
			P2	Ф = 10 ± 0 mm
			P5	Ф = 9.5 ± 0.4 mm
Aerial parts	Essential oil	Agar diffusion methods, broth microdilution assay	Staphylococcus aureus	Ф = 35.67 ± 0.66 mm	[3]
				MIC = 0.03%
				MMC = 0.13%
			Escherichia coli	Ф = 26.33 ± 1.66 mm
				MIC = 0.03%
				MMC = 0.03%
			Pseudomonas aeruginosa	Ф = 9.33 ± 0.66 mm
				MIC = 0.5%
				MMC = 0.5%
Leaves	Ethanolic extract	Agar-well diffusion assay	Escherichia coli	Ф = 30.33 ± 2.51 mm	[11]
Leaves	Essential oil	Disk-diffusion assay; Microtitration method	Salmonella CECT 915	Ф = 34.33 ± 4.04 mm	[6]
				MIC = 0.0625%
				MBC = 0.0625%
			Salmonella S64	Ф = 28.17 ± 1.61 mm
				MIC = 0.0625%
				MBC = 0.125%
			E. coli O157 : H7 CECT4267	Ф = 19.67 ± 1.15 mm
				MIC = 0.25%
				MBC = 0.5%
			L. monocytogenes CECT4031	Ф = 34.00 ± 0.00 mm
				MIC = 0.125%
				MBC = 0.0625%
			L. monocytogenes L23	Ф = 31.00 ± 3.46 mm
				MIC = 0.5%
				MBC = 0.5%
Flowering tops	Essential oil	Disk-diffusion assay; Microtitration method	Salmonella CECT 915	Ф = 31.50 ± 2.78 mm	[6]
				MIC = 0.0625
				MBC = 0.125%
			Salmonella S64	Ф = 28.17 ± 1.61 mm
				MIC = 0.0625%
				MBC = 0.125%
			E. coli O157 : H7 CECT4267	Ф = 18.00 ± 0.00 mm
				MIC = 0.25%
				MBC = 0.5%
			L. monocytogenes CECT4031	Ф = 29.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.25%
			L. monocytogenes L23	Ф = 31.00 ± 3.46 mm
				MIC = 0.5%
				MBC = 0.5%
Flowering tops	Essential oil		Total mesophilic aerobic flora (FMAT)	Significant effect on microbial growth	[10]
Aerial parts	Essential oil	Agar-well diffusion method; Microdilution assay	E. coli ATCC 25922	Ф = 21.33 ± 0.57 mm	[4]
				MIC = 0.5%
				MBC < 1%
			E. coli K12	Ф = 16.00 ± 1.00 mm
				MIC = 0.25%
				MBC = 0.5%
			B. subtilis DCM 6633	Ф = 24.66 ± 1.52 mm
				MIC = 0.5%
				MBC = 0.5%
			S. aureus ATCC 25923	Ф = 27.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.125%
Aerial parts	Dissolved volatile fraction	Agar-well diffusion method; Microdilution assay	E. coli ATCC 25922	Ф = 28.33 ± 0.57 mm	[4]
				MIC = 0.125%
				MBC = 0.25%
			E. coli K12	Ф = 17.00 ± 1.73 mm
				MIC = 0.125%
				MBC = 0.125%
			B. subtilis DCM 6633	Ф = 41.00 ± 2.64 mm
				MIC = 0.0625%
				MBC = 0.0625%
			S. aureus ATCC 25923	Ф = 30.00 ± 2.00 mm
				MIC = 0.0312%
				MBC = 0.0312%