Effects of Water Extract of Cynanchum paniculatum (Bge.) Kitag. on Different Breast Cancer Cell Lines

Table 3

Differential sensitivity to cytotoxicity is correlated to genomic characteristics.

Breast cancer cell lines	ER	PR	HER	5 mg/ml; 24 hr cytotoxicity less than 20%	3 mg/ml; 72 hr cytotoxicity less than 30%
MDA-MB-231	−	−	−	Y	Y
SK-Br-3	−	−	+	Y	Y
MCF-7	+	+	−	N	N
ZR-75-1	+	+	+	N	NA

The different genomic characteristics were listed in an array and compared to two traits (shown in the last two columns). The first result column demonstrated that when the cells were treated with 5mg/ml CP extract, MCF−7 and ZR−75−1 (both ER(+)PR(+)) demonstrated about 40-50% survival, hence the N (no) for this phenotype, while MDA-MB-231 and SK−Br−3 (both ER(−)PR(−)), demonstrated about 10% survival, hence the Y (yes). The second result column summarizes the results whether this cell line could be killed significantly to be less than 30% of control group with 3 mg/ml CP after 72 hours of incubation. The two ER(−)PR(−) cells were sensitive (hence the Y result) while the two ER(+)PR(+) cells are not. For details of the results, please see Figure 2 and respective text.