Research Article

Anticancer Activity of Ipomoea purpurea Leaves Extracts in Monolayer and Three-Dimensional Cell Culture

Figure 7

Cleavage of caspase-9 and caspase-8 processing in A-549 cell line upon exposure with DMSO 0.1% (negative control), IC50 values of etoposide (positive control) and methanol and chloroform extracts of I. purpurea for 24 h. Following treatment, the cells were lysed and analyzed by western blot with anti-caspase-9 and anti-caspase-8 antibodies. β-Actin (43 KD) was used to confirm equal protein loading. (a) The molecular weight of procaspase-9 and activated (cleaved) caspase-9 was 46 and 37 kD, respectively. (b) The molecular weight of procaspase-8 was 57 kD. (c) Semiquantitative analysis of western blot for caspase-9. The intensity of each band was quantified by ImageJ image processing program. All values were expressed as mean ± SD of at least three determinations. Significant differences are indicated by  < 0.05 and  < 0.01 as compared with the control group.
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