Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice

Figure 2

AG inhibited the production of cytokines expressed by Th17 cells and the expression of transcription factor induced by Th17 cells in OVA-stimulated asthmatic mice. Animals were randomly divided into 4 groups, sensitized by intraperitoneal injection of OVA (0.1 μg/μL) and intraperitoneally administrated AG (0.1 mg/ml) 1 h before each challenge with atomized OVA. The serum and BALF were collected and used to detect the concentration of IL-6, IL-17A, and IL-17F separately. The production of IL-6, IL-17A, and IL-17F was inhibited by AG (a–f). The lung tissues were collected and used to detect the mRNA expression of RORα and RORγt. The mRNA expression of RORγt (g) was inhibited by AG. All values are expressed as the mean ± the standard deviation of triplicate tests. relative to the control group; , relative to the OVA group.

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