Anti-Inflammatory and Anti-Vascular Leakage Effects by Combination of Centella asiatica and Vitis vinifera L. Leaf Extracts

Figure 2

Effects of CE, VVE, or CE-VVE 1 : 3 combination on NO and PGE₂ production in LPS-stimulated RAW264.7 cells. (a) Cells were plated and cultured for 24 h in the absence or presence of different concentrations of CE, VVE, and CE-VVE 1 : 3 combination. Cells were pretreated with CE, VVE, or CE-VVE 1 : 3 combination (0, 5, 10, 15, 20, 25, and 30 μg/mL) for 1 h and then stimulated with LPS (500 ng/mL) for 24 h. NO production was calculated in culture media by the Griess reagent. (b) PGE₂ production was quantified by ELISA. All results (n = 3) are expressed as the mean ± SD, , vs. LPS-stimulated cells. Abbreviations: CE, Centella asiatica extract; LPS, lipopolysaccharide; NO, nitric oxide; PGE₂, prostaglandin E₂; VVE, Vitis vinifera (L.) extract.

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