Shu-Di-Huang and Gan-Cao Herb Pair Restored the Differentiation Potentials of Mesenchymal Stem Progenitors in Treating Osteoporosis via Downregulation of NF-κB Signaling Pathway

Figure 3

RR-L containing serum improved osteogenesis activity and suppressed adipogenesis activity in MSCs via inhibiting NF-κB signaling pathway in a dose-dependent manner in vitro. (a) MTT assay revealed that 10%–15% RR-L containing serum promoted MSC proliferation in a dose-dependent manner. (b) Alizarin red staining revealed that RR-L containing serum improved osteogenesis activity in a dose-dependent manner. (c) RR-L containing serum suppressed adipogenesis activity in a dose-dependent manner. (d–f) RR-L containing serum inhibited NF-κB signaling pathway in a dose-dependent manner. (g) Statistical result of (b). (h) Statistical result of (c). (i) Statistical result of (d–f). (j) Representative western blots image of PPARγ, RUNX2, OCN, p65, and β-actin. (k–n) Statistical results of western blot via Image Lab software. versus control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)