Shu-Di-Huang and Gan-Cao Herb Pair Restored the Differentiation Potentials of Mesenchymal Stem Progenitors in Treating Osteoporosis via Downregulation of NF-κB Signaling Pathway

Figure 5

Cotreatment of NF-κB signaling pathway inhibitor BAY117082 and RR-L containing serum showed no better effect than BAY117082 along on MSCs in vitro. No differences were observed between RR-L containing serum and BAY117082 in (a) osteogenesis staining; (b) adipogenesis staining, and (c-e) NF-κB signaling pathway activation of MSCs. (f–h) Statistical results of Alizarin red, Oil red staining, and IF analysis. (i) Representative western blots image of PPARγ, RUNX2, OCN, p65, and β-actin. (j–m) Statistical results of western blot via Image Lab software. (n–q) mRNA expressions of PPARγ, RUNX2, OCN, and p65. versus ctrl, NS, versus BAY117082 group.

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