Samchulkunbi-Tang Alleviates Vascular Endothelial Disorder and Renal Dysfunction in Nitric Oxide-Deficient Hypertensive Rats

Figure 2

Effect of SCT on NO production, phosphorylated eNOS, and Akt in HUVEC. (a) Effect of SCT on the viability of cells (HUVEC). SCT (0–100 μg/mL) was treated for 24 hours. (b) NO production was investigated through a fluorescence microscope (magnification, ×100). (c) The nitric oxide production of each group was analyzed as a Griess reaction. (d) Effect of SCT on p-eNOS expression was confirmed by western blot. (e) Effect of SCT on p-Akt expression was analyzed by western blot. (f) Effect of SCT on the expression of vascular inflammatory factors in HUVECs. SCT was pretreated for 30 minutes and then stimulated with TNF-α (50 ng/mL). Protein expression of inflammatory factors (ICAM-1, VCAM-1, and E-selectin) was confirmed using Western blotting. Values are expressed as mean ± S.E. Student’s t-test on paired or unpaired data was also applied. All statistical analyses were conducted using Sigma Plot 10. ^∗∗ vs. CONT, ^## vs. SCT 1 μg/mL, and ^$$ vs. SCT 5 μg/mL.

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